Respiratory Syncytial virus type A Nucleoprotein, DDDDK tag

Cat. No. GTX136751-pro

Application	WB, ELISA, Lateral Flow, Sandwich ELISA
Species	Respiratory syncytial virus type A
Package	100 μg ($499)

See all Respiratory syncytial virus Nucloprotein products

Datasheet

APPLICATION

Application Note

Recommended antibody pairs for sandwich ELISA:
Capture: GTX636711 / GTX636710 / GTX636709, Detection: GTX636649 or Capture: GTX636649 / GTX636650, Detection: GTX636648.
Please notice that the detected antibody needs to be conjugated to HRP to function when paired with capture antibodies. Please contact us for custom HRP-conjugated antibody.

PROPERTIES

Form

Liquid

Buffer

TBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

Respiratory syncytial virus of YP_009518852.1 (1-391 a.a) with DDDDK tag at the C-terminus

Expression System

HEK293 cells

Purity

>95%

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

GTX136751-pro ELISA Image

Sandwich ELISA detection of recombinant HEK293 expressed, full-length respiratory syncytial virus type A nucloprotein, DDDDK tag (GTX136751-pro) using ELISA pairs below.
Pair 1:
Capture: Respiratory Syncytial virus type A Nucleoprotein antibody [HL1297] (GTX636711) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP (1 μg/mL)
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1296] (GTX636710) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) conjugated with HRP (1 μg/mL)
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

GTX136751-pro ELISA Image

Sandwich ELISA of recombinant nucleoproteins (NP) derived from different strains of respiratory syncytial virus (i.e., type A; type B) using ELISA pairs below.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP (1 μg/mL)
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP (1 μg/mL)
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

GTX136751-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant nucleoproteins (NP) derived from different strains of respiratory syncytial virus (i.e., type A; type B) (84.76-1.32 nM). Coated protein was probed with Respiratory Syncytial virus Nucleoprotein antibodies (1 μg/mL) below. Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
Antibodies:
1. Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650)
2. Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649)
3. Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (GTX636648)

GTX136751-pro ELISA Image

Sandwich ELISA detection of recombinant Respiratory syncytial virus type A Nucleoprotein, DDDDK tag (GTX136751-pro) using antibodies as below.

Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1295] (GTX636709) (5 μg/mL)
Detection: HRP-conjugated Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) (1 μg/mL)
Please notice that GTX636649 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636709. Please contact us for custom HRP-conjugated antibody.

GTX136751-pro ELISA Image

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of respiratory syncytial virus (i.e., type A; type B) using antibodies as below.

Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) (5 μg/mL)
Detection: HRP-conjugated Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648) (1 μg/mL)
Please notice that GTX636648 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636650. Please contact us for custom HRP-conjugated antibody.

GTX136751-pro ELISA Image

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of respiratory syncytial virus (i.e., type A; type B) using antibodies as below.

Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) (5 μg/mL)
Detection: HRP-conjugated Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648) (1 μg/mL)
Please notice that GTX636648 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636649. Please contact us for custom HRP-conjugated antibody.

GTX136751-pro ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant Respiratory syncytial virus type A Nucleoprotein, DDDDK tag (GTX136751-pro) (84.76-1.32 nM). Coated protein was probed with the specified respiratory syncytial virus nucleoprotein antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

GTX136751-pro ELISA Image

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of respiratory syncytial virus (i.e., type A; type B) using antibodies as below.

Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1245] (GTX636647) (5 μg/mL)
Detection: HRP-conjugated Respiratory Syncytial virus Nucleoprotein antibody [HL1291] (GTX636705) (1 μg/mL)
Please notice that GTX636705 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636647. Please contact us for custom HRP-conjugated antibody.

GTX136751-pro Lateral Flow Image

Detection of respiratory syncytial virus type A nucleoprotein (GTX136751-pro) (0.5 μg/ml) by lateral flow assay using the recombinant rabbit monoclonal antibody pair.

GTX136751-pro Image

GTX136751-pro Respiratory syncytial virus type A Nucloprotein, DDDDK tag protein were analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

GTX136751-pro Image

Detection of Respiratory Syncytial virus Nucleoprotein by lateral flow assay using the monoclonal antibody pair.

Capture: Respiratory Syncytial virus Nucleoprotein antibody (GTX636650) [HL1248])
Detection: Respiratory Syncytial virus Nucleoprotein antibody (GTX636648) [HL1246])

Samples (25 ng) :
1. RSV type A Nucleoprotein (GTX136751-pro)
2. RSV type B Nucleoprotein (GTX136931-pro)
3. SARS-CoV-2 Nucleocapsid protein (GTX135592-pro)
4. FluA (H1N1) Nucleoprotein (GTX135904-pro)
5. FluA (H3N2) Nucleoprotein (GTX135903-pro)
6. FluB Nucleoprotein (GTX136277-pro)
7. Lysis buffer

GTX136751-pro WB Image

Respiratory Syncytial virus type A Nucleoprotein, DDDDK tag (1 μg, GTX136751-pro) were separated by 12% SDS-PAGE, and the membrane was blotted with DDDDK tag antibody [GT1216] (GTX636630) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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Package	List Price ($)
100 μg	$ 499
Qty

Respiratory Syncytial virus type A Nucleoprotein, DDDDK tag

Cat. No. GTX136751-pro

Application

Species

APPLICATION

Application Note

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Region/Sequence

Expression System

Purity

Note

TARGET

Research Area

DATA IMAGES

GTX136751-pro ELISA Image

GTX136751-pro ELISA Image

GTX136751-pro ELISA Image

GTX136751-pro ELISA Image

GTX136751-pro ELISA Image

GTX136751-pro ELISA Image

GTX136751-pro ELISA Image

GTX136751-pro ELISA Image

GTX136751-pro ELISA Image

GTX136751-pro Lateral Flow Image

GTX136751-pro Image

GTX136751-pro Image

GTX136751-pro WB Image

REFERENCE

REVIEW

Product

Research

Support

Company

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