Respiratory Syncytial virus type B Nucleoprotein, DDDDK tag

Cat. No. GTX136931-pro

Application	WB, ELISA, Sandwich ELISA
Species	Respiratory syncytial virus type B
Package	100 μg ($499)

See all Respiratory Syncytial virus type B Nucleoprotein products

Datasheet

APPLICATION

Application Note

Recommended antibody pairs for sandwich ELISA:
Capture: GTX636649 / GTX636650, Detection: GTX636648.
Please notice that the detected antibody needs to be conjugated to HRP to function when paired with capture antibodies. Please contact us for custom HRP-conjugated antibody.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.45 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

Full length Respiratory Syncytial virus type B Nucleoprotein with a C-terminal DDDDK tag (strain B1).

Expression System

HEK293 cells

Purity

>95%

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

GTX136931-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant nucleoproteins (NP) derived from different strains of respiratory syncytial virus (i.e., type A; type B) (84.76-1.32 nM). Coated protein was probed with Respiratory Syncytial virus Nucleoprotein antibodies (1 μg/mL) below. Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
Antibodies:
1. Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650)
2. Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649)
3. Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (GTX636648)

GTX136931-pro ELISA Image

Sandwich ELISA of recombinant nucleoproteins (NP) derived from different strains of respiratory syncytial virus (i.e., type A; type B) using ELISA pairs below.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP (1 μg/mL)
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP (1 μg/mL)
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

GTX136931-pro ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant Respiratory Syncytial virus type B Nucleoprotein, DDDDK tag (GTX136931-pro) (84.76-1.32 nM). Coated protein was probed with the specified respiratory syncytial virus nucleoprotein antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

GTX136931-pro ELISA Image

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of respiratory syncytial virus (i.e., type A; type B) using antibodies as below.

Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1245] (GTX636647) (5 μg/mL)
Detection: HRP-conjugated Respiratory Syncytial virus Nucleoprotein antibody [HL1291] (GTX636705) (1 μg/mL)
Please notice that GTX636705 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636647. Please contact us for custom HRP-conjugated antibody.

GTX136931-pro Image

GTX136931-pro Respiratory Syncytial virus type B Nucleoprotein, DDDDK tag protein were analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

GTX136931-pro WB Image

Respiratory Syncytial virus type B Nucleoprotein, DDDDK tag (1 μg, GTX136931-pro) were separated by 12% SDS-PAGE, and the membrane was blotted with DDDDK tag antibody [GT1216] (GTX636630) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX136931-pro Image

Detection of Respiratory Syncytial virus Nucleoprotein by lateral flow assay using the monoclonal antibody pair.

Capture: Respiratory Syncytial virus Nucleoprotein antibody (GTX636650) [HL1248])
Detection: Respiratory Syncytial virus Nucleoprotein antibody (GTX636648) [HL1246])

Samples (25 ng) :
1. RSV type A Nucleoprotein (GTX136751-pro)
2. RSV type B Nucleoprotein (GTX136931-pro)
3. SARS-CoV-2 Nucleocapsid protein (GTX135592-pro)
4. FluA (H1N1) Nucleoprotein (GTX135904-pro)
5. FluA (H3N2) Nucleoprotein (GTX135903-pro)
6. FluB Nucleoprotein (GTX136277-pro)
7. Lysis buffer

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Package

List Price ($)

100 μg

$ 499

Qty

Respiratory Syncytial virus type B Nucleoprotein, DDDDK tag

Cat. No. GTX136931-pro

Application

Species

APPLICATION

Application Note

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Region/Sequence

Expression System

Purity

Conjugation

Note

TARGET

Research Area

DATA IMAGES

GTX136931-pro ELISA Image

GTX136931-pro ELISA Image

GTX136931-pro ELISA Image

GTX136931-pro ELISA Image

GTX136931-pro Image

GTX136931-pro WB Image

GTX136931-pro Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.