Sandwich ELISA of recombinant nucleoproteins (NP) derived from different strains of respiratory syncytial virus (i.e., type A; type B) using ELISA pairs below.
Pair 1:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP (1 μg/mL)
Pair 2:
Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649) (5 μg/mL)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL1246] (GTX636648) conjugated with HRP (1 μg/mL)
Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture one.
Please contact us for custom HRP-conjugated antibody.

Indirect ELISA analysis performed by coating plate with recombinant nucleoproteins (NP) derived from different strains of respiratory syncytial virus (i.e., type A; type B) (84.76-1.32 nM). Coated protein was probed with Respiratory Syncytial virus Nucleoprotein antibodies (1 μg/mL) below. Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
Antibodies:
1. Respiratory Syncytial virus Nucleoprotein antibody [HL1248] (GTX636650)
2. Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649)
3. Respiratory Syncytial virus type A Nucleoprotein antibody [HL1246] (GTX636648)

Detection of Respiratory Syncytial virus Nucleoprotein by lateral flow assay using the monoclonal antibody pair.

Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1247] (GTX636649)
Detection: Respiratory Syncytial virus Nucleoprotein antibody [HL2182] (GTX638186)

Samples (40 ng) :
1. Running buffer (N)
2. Respiratory Syncytial virus type A Nucleoprotein (GTX136751-pro)
3. Respiratory Syncytial virus type B Nucleoprotein (GTX136931-pro)

Detection of nucleoproteins (NP) derived from respiratory syncytial virus, SARS-CoV-2, Influenza A virus and Influenza B virus by lateral flow assay using the monoclonal antibody pair.

Capture: Respiratory Syncytial virus Nucleoprotein antibody (GTX638184 [HL2180])
Detection: Respiratory Syncytial virus Nucleoprotein antibody (GTX638186 [HL2182])

Samples (40 ng) :
1. Running buffer
2. Respiratory Syncytial virus type A Nucleoprotein (GTX136751-pro)
3. Respiratory Syncytial virus type B Nucleoprotein (GTX136931-pro)
4. SARS-CoV-2 (COVID-19) Nucleocapsid protein, Omicron / BA.2 / BA.5 variant, His tag (GTX137219-pro)
5. SARS-CoV-2 (COVID-19) Nucleocapsid protein, Omicron / BQ.1 variant, His tag (GTX137883-pro)
6. Influenza A virus Nucleoprotein (A/Hong Kong/2671/2019 (H3N2)) (GTX136317-pro)
7. Influenza A virus Nucleoprotein (A/California/2009 (H1N1)) (GTX135904-pro)
8. Influenza B virus Nucleoprotein (B/Sydney/3/2004) (GTX135867-pro)
9. Influenza B virus Nucleoprotein (B/Victoria/02/1987) (GTX136277-pro)

Detection of Respiratory Syncytial virus Nucleoprotein by lateral flow assay using the monoclonal antibody pair.

Capture: Respiratory Syncytial virus Nucleoprotein antibody (GTX636650) [HL1248])
Detection: Respiratory Syncytial virus Nucleoprotein antibody (GTX636648) [HL1246])

Samples (100 ng) :
1. RSV type A Nucleoprotein (GTX136751-pro)
2. RSV type B Nucleoprotein (GTX136931-pro)
3. SARS-CoV-2 Nucleocapsid protein (GTX135592-pro)
4. FluA (H1N1) Nucleoprotein (GTX135904-pro)
5. FluA (H3N2) Nucleoprotein (GTX135903-pro)
6. FluB Nucleoprotein (GTX136277-pro)
7. Lysis buffer

Sandwich ELISA detection of recombinant nucleoproteins (NP) derived from different strains of respiratory syncytial virus (i.e., type A; type B) using antibodies as below.

Capture: Respiratory Syncytial virus Nucleoprotein antibody [HL1245] (GTX636647) (5 μg/mL)
Detection: HRP-conjugated Respiratory Syncytial virus Nucleoprotein antibody [HL1291] (GTX636705) (1 μg/mL)
Please notice that GTX636705 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636647. Please contact us for custom HRP-conjugated antibody.

Indirect ELISA analysis was performed by coating the plate with recombinant Respiratory Syncytial virus type B Nucleoprotein, DDDDK tag (GTX136931-pro) (84.76-1.32 nM). Coated protein was probed with the specified respiratory syncytial virus nucleoprotein antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

Respiratory Syncytial virus type B Nucleoprotein, DDDDK tag (1 μg, GTX136931-pro) were separated by 12% SDS-PAGE, and the membrane was blotted with DDDDK tag antibody [GT1216] (GTX636630) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX136931-pro Respiratory Syncytial virus type B Nucleoprotein, DDDDK tag protein were analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.