Wild-type (WT) and S100A11 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with S100A11 antibody (GTX100698) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using S100A11(GTX100698) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded SW480 Xenograft, using S100A11(GTX100698) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min