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SA1 antibody

Anti-SA1 antibody used in Western Blot (WB). GTX130364
Anti-SA1 antibody used in Immunoprecipitation (IP). GTX130364
Anti-SA1 antibody used in Western Blot (WB). GTX130364

Cat No. GTX130364

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

144 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human SA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

stromal antigen 1,MRD47,SA1,SCC3A

Cellular Localization

Nucleus

Background

This gene is a member of the SCC3 family and is expressed in the nucleus. It encodes a component of cohesin, a multisubunit protein complex that provides sister chromatid cohesion along the length of a chromosome from DNA replication through prophase and prometaphase, after which it is dissociated in preparation for segregation during anaphase. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-SA1 antibody used in Western Blot (WB). GTX130364

GTX130364 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody (GTX130364) diluted at 1:3000.

Anti-SA1 antibody used in Immunoprecipitation (IP). GTX130364

GTX130364 IP Image

Immunoprecipitation of SA1 protein from HeLa whole cell extracts using 5 μg of SA1 antibody (GTX130364).
Western blot analysis was performed using SA1 antibody (GTX130364).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-SA1 antibody used in Western Blot (WB). GTX130364

GTX130364 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with SA1 antibody (GTX130364) diluted at 1:1000.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169