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SAFB antibody [6F7]

Anti-SAFB antibody [6F7] used in Western Blot (WB). GTX70197
Anti-SAFB antibody [6F7] used in Western Blot (WB). GTX70197
Anti-SAFB antibody [6F7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70197

Cat No. GTX70197

Host

Mouse

Clonality

Monoclonal

Clone Name

6F7

Isotype

IgG1

Application

WB, ICC/IF, IP, IHC

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/ml
ICC/IF Assay dependent
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

Positive Control

HeLa, MCF7

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to amino acids 345-357 (PEARDSKEDGRKF) of the human SAFB protein.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Scaffold Attachment Factor B , Hap , Het , Sab-B1 , Saf-B , Saf-B1 , Safb1 , Safb

Background

Hsp27 ERE-TATA binding protein (HET)/ Scaffold Attachment Factor-B (SAF-B) is a member of a subset of nuclear matrix proteins that mediate the attachment of chromatin to nuclear protein complexes. The calculated molecular weight of HET/SAF-B is 102 kDa (Accession No. U72355), but on SDS PAGE gels HET/SAF-B runs as an approximately 130 kDa protein. This aberrant migration is due to a large number of charged amino acid residues.

Database

Research Area

DATA IMAGES

Anti-SAFB antibody [6F7] used in Western Blot (WB). GTX70197

GTX70197 WB Image

HET/SAF-B antibody [6F7] detects HET/SAF-B protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
5% SDS-PAGE
HET/SAF-B antibody [6F7] (GTX70197) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SAFB antibody [6F7] used in Western Blot (WB). GTX70197

GTX70197 WB Image

Detection of human HET SAF-B protein in HeLa whole cell lysate using GeneTex monoclonal antibody GTX70197

Anti-SAFB antibody [6F7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70197

GTX70197 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HET/SAF-B [6F7](GTX70197) antibody at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329