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SAFB antibody [6F7]

Anti-SAFB antibody [6F7] used in Western Blot (WB). GTX70197
Anti-SAFB antibody [6F7] used in Western Blot (WB). GTX70197
Anti-SAFB antibody [6F7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70197

Cat. No. GTX70197

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6F7

Isotype

 IgG1

Application

 WB, ICC/IF, IP, IHC

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all SAFB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/ml
ICC/IF Assay dependent
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

103 kDa. ( Note )

Positive Control

HeLa, MCF7

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to amino acids 345-357 (PEARDSKEDGRKF) of the human SAFB protein.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_371724

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

scaffold attachment factor B , HAP , HET , SAB-B1 , SAF-B , SAF-B1 , SAFB1

Cellular Localization

Nucleus

Background

This gene encodes a DNA-binding protein which has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. The encoded protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of heat shock protein 27 transcription, can act as an estrogen receptor co-repressor and is a candidate for breast tumorigenesis. This gene is arranged head-to-head with a similar gene whose product has the same functions. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2011]

Database

Research Area

DATA IMAGES

Anti-SAFB antibody [6F7] used in Western Blot (WB). GTX70197

GTX70197 WB Image

HET/SAF-B antibody [6F7] detects HET/SAF-B protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
5% SDS-PAGE
HET/SAF-B antibody [6F7] (GTX70197) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SAFB antibody [6F7] used in Western Blot (WB). GTX70197

GTX70197 WB Image

Detection of human HET SAF-B protein in HeLa whole cell lysate using GeneTex monoclonal antibody GTX70197

Anti-SAFB antibody [6F7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70197

GTX70197 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HET/SAF-B [6F7](GTX70197) antibody at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations