ICC/IF analysis of RSC-96 cells using GTX23437 SAP97 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature

ICC/IF analysis of C2C12 cells using GTX23437 SAP97 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC

ICC/IF analysis of HeLa cells using GTX23437 SAP97 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC

ICC/IF analysis of NIH-3T3 cells using GTX23437 SAP97 antibody. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:100 incubated overnight at 4 ºC

WB analysis of 25 ug of C6 (lane 1), PC12 (lane 2) and rat brain (lane 3) cell lysates using GTX23437 SAP97 antibody.
Dilution : 1:5000