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SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU]

Recombinant antibody
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] used in Neutralizing/Blocking/Inhibition (Neutralizing/Inhibition). GTX635866
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] used in ELISA (ELISA). GTX635866
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] used in ELISA (ELISA). GTX635866

Cat. No. GTX635866

Reference 2

Host

 Human

Clonality

 Monoclonal

Clone Name

 HL1003-HU

Isotype

 IgG

Application

 ELISA, Neutralizing/Inhibition, Sandwich ELISA

Reactivity

 SARS Coronavirus 2
Package
100 μl ($399),
25 μl ($169)
See all SARS-CoV-2 (COVID-19) Spike products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ELISA Assay dependent
Neutralizing/Inhibition Assay dependent
Sandwich ELISA Assay dependent

Note :

Neutralizing/Inhibition
This antibody inhibits infection of mammalian cells by live SARS-CoV-2.

Sandwich ELISA
Capture : GTX635807, Detection: GTX635866.
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Recombinant protein corresponding to SARS-CoV-2 (COVID-19) Spike RBD (SARS-CoV-2 (strain Wuhan-Hu-1)). The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888584

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Database

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] used in Neutralizing/Blocking/Inhibition (Neutralizing/Inhibition). GTX635866

GTX635866 Neutralizing/Inhibition Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX136090-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] (GTX635866) (0.09-66.67 nM). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] used in ELISA (ELISA). GTX635866

GTX635866 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] (GTX635866) (1 μg/mL). Human IgG antibody (HRP) (1:200000) was used to detect bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] used in ELISA (ELISA). GTX635866

GTX635866 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] (GTX635866) (1 μg/mL)Anti-Human IgG antibody (HRP) was diluted at 1:200000 and used to detect the primary antibody.

REFERENCE

Application Reference

W-F Tang et al. Antimicrob Agents Chemother 2024; : e0095623 BPR3P0128, a non-nucleoside RNA-dependent RNA polymerase inhibitor, inhibits SARS-CoV-2 variants of concern and exerts synergistic antiviral activity in combination with remdesivir
Application : Neutralizing/Inhibition
Cheedarla N et al. iScience 2023; 26 (11) : 108256 Rapid, high throughput, automated detection of SARS-CoV-2 neutralizing antibodies against Wuhan-WT, delta and omicron BA1, BA2 spike trimers.
Application : ELISA
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Package List Price ($)
$ 399
$ 169
Custom Conjugations