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SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU]

Recombinant antibody
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] used in ELISA (ELISA). GTX635866
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] used in ELISA (ELISA). GTX635866
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] used in ELISA (ELISA). GTX635866
Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] used in Neutralizing/Blocking/Inhibition (Neutralizing/Inhibition). GTX635866

Cat No. GTX635866

Host

Human

Clonality

Monoclonal

Clone Name

HL1003-HU

Isotype

IgG

Application

ELISA, Neutralizing/Inhibition, Sandwich ELISA

Reactivity

SARS Coronavirus 2
Package
100 μl ($369),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ELISA Assay dependent
Neutralizing/Inhibition Assay dependent
Sandwich ELISA Assay dependent

Note :

Neutralizing/Inhibition
This antibody inhibits infection of mammalian cells by live SARS-CoV-2.

Sandwich ELISA
Capture : GTX635807, Detection: GTX635866.

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Recombinant protein corresponding to SARS-CoV-2 (COVID-19) Spike RBD (SARS-CoV-2 (strain Wuhan-Hu-1)). The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888584

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] used in ELISA (ELISA). GTX635866

GTX635866 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Spike ECD protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B1.617.2 delta variant) (29.71-0.46 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] (GTX635866) (1 μg/mL). Human IgG antibody (HRP) (1:200000) was used to detect bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] used in ELISA (ELISA). GTX635866

GTX635866 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) protein (GTX135817-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] (GTX635866) as detection antibody at concentration of 1 μg/mL. Human IgG antibody (HRP) was diluted at 1:200000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] used in ELISA (ELISA). GTX635866

GTX635866 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] (GTX635866) as detection antibody at concentration of 1 μg/mL. Human IgG antibody (HRP) was diluted at 1:200000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] used in Neutralizing/Blocking/Inhibition (Neutralizing/Inhibition). GTX635866

GTX635866 Neutralizing/Inhibition Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX136090-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003-HU] (GTX635866) (0.09-66.67 nM). Bound ACE2 was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

REFERENCE

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REVIEW

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