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SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active)

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro
SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro
SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro
SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro
SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro
SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro
SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro
SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro
SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro
SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro
SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro
SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro
SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro

Cat. No. GTX01546-pro

Reference 2

Application

 ELISA, Functional Assay

Species

 SARS Coronavirus 2
Package
100 μg ($539)
See all SARS-CoV-2 (COVID-19) Spike products

APPLICATION

Application Note

Activity was measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 (COVID-19) Spike RBD protein at 2 μg/mL (100 μL/well) can bind Recombinant Human ACE2 with a linear range of 8-80 ng/mL.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with distilled water to 0.1-0.5mg/ml. Lyophilized from 0.22μm-filtered PBS (pH7.4).

Preservative

No preservative

Storage

Store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

Arg319-Phe541 of SARS-CoV-2 (COVID-19) Spike RBD protein (Accession #YP_009724390.1) fused with a 6×His tag at the C-terminus.

Expression System

HEK293 cells

Purity

> 95% by SDS-PAGE

Endotoxin

< 0.1 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SARS-CoV-2 Spike , COVID-19 Spike

Database

Research Area

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro

GTX01546-pro ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (HRP) (GTX635793-01) as detection antibody at concentration of 1 μg/mL.

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro

GTX01546-pro ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (HRP) (GTX635792-01) as detection antibody at concentration of 1 μg/mL.

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro

GTX01546-pro Functional Assay Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (4.5-10000 ng/mL). Bound protein was detected by ACE2 antibody [N1N2], N-term (GTX101395) (0.5 μg/mL) and Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro

GTX01546-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (50 ng). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) (1000-1.19*10-4 μM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
EC50 : 0.24 μM

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro

GTX01546-pro ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1002] (GTX635791) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635791 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635792. Please contact us for custom HRP-conjugated antibody.

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro

GTX01546-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (50 ng). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (1000-1.19*10-4 μM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
EC50 : 0.2 μM

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro

GTX01546-pro Functional Assay Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417T, E484K, N501Y Mutant) protein, His tag (active) (GTX136043-pro), SARS-CoV-2 (COVID-19) Spike RBD (E484K, N501Y Mutant) protein, His tag (active) (GTX136058-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (13-10000 ng/mL). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro

GTX01546-pro Functional Assay Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, mouse Fc tag (UK variant) (GTX136024-pro) and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (active) (GTX135683-pro) (13.72-10000 ng/mL). Bound protein was detected by ACE2 antibody [N1N2], N-term (GTX101395) (0.1 μg/mL), and Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro

GTX01546-pro ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) and SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro) using SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1014] (GTX635807) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635793 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635807. Please contact us for custom HRP-conjugated antibody.

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro

GTX01546-pro Functional Assay Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (14-10000 ng/mL). Bound ACE2 protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro

GTX01546-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (50 ng). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1003] (GTX635792) (160-1.91*10-5 μM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.
EC50 : 0.08 μM

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro

GTX01546-pro Functional Assay Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) (GTX136014-pro), SARS-CoV-2 (COVID-19) Spike RBD (K417N, E484K, N501Y Mutant) protein, His tag (active) (GTX136022-pro), and SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody [HL1004] (GTX635793) (14-10000 ng/mL). Bound ACE2 protein was detected by Goat Anti-Mouse IgG antibody (HRP) (13111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active). GTX01546-pro

GTX01546-pro Image

SDS-PAGE of GTX01546-pro SARS-CoV-2 (COVID-19) Spike RBD protein (active).

REFERENCE

Application Reference

TW Liu et al. J Ethnopharmacol 2024; 320 : 117412 Polymethoxyflavone from Citrus depressa as an inhibitor against various variants of SARS-CoV-2 spike protein
Ganneru B. et al. iScience 2021; 24 (4) : 102298 Th1 skewed immune response of whole virion inactivated SARS CoV 2 vaccine and its safety evaluation.
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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 539