Activity was measured by its binding ability in a functional ELISA. ImmobilizedSARS-CoV-2 (COVID-19) Spike S1 protein at 0.5 μg/mL (100 μL/well) can bind Recombinant Human ACE2 with a linear range of 0.06-0.25 μg/mL.
PROPERTIES
Form
Lyophilized powder
Buffer
Reconstitute with distilled water to a concentration of 0.1-0.5mg/ml. Lyophilized from 0.22μm-filtered PBS (pH7.4).
Preservative
No preservative
Storage
Store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
Region/Sequence
Gln14-Arg683 of SARS-CoV-2 (COVID-19) Spike S1 protein (Accession #YP_009724390.1) fused with a 6×His tag and Avi at the C-terminus.
Expression System
HEK293 cells
Purity
> 95% by SDS-PAGE
Endotoxin
< 0.1 EU/μg (determined by LAL method)
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His and Avi tag (active) protein (GTX01548-pro) (4000-62.5 ng/mL). Coated protein probed with SARS-CoV-2 (COVID-19) Spike antibody (GTX135360) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
Show More +
GTX01548-pro ELISA Image
Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 protein (GTX01548-pro) (62.5-4000 ng/mL). Coated protein probed with SARS-CoV-2 (COVID-19) Spike antibody (GTX135356) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
Show More +
GTX01548-pro Functional Assay Image
Inhibition ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His and Avi tag (active) (GTX01548-pro) (coated at 2 μg/mL) inhibiting by SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) (14-10000 ng/mL), and binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (3000 ng/mL). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).
Show More +
GTX01548-pro Functional Assay Image
Functional ELISA analysis of immobilized GTX01548-pro SARS-CoV-2 (COVID-19) Spike S1 protein (active) at 0.5μg/mL (100 μL/well) can bind recombinant human ACE2 with a linear range of 0.06-0.25μg/mL.
Show More +
GTX01548-pro Image
SDS-PAGE of GTX01548-pro SARS-CoV-2 (COVID-19) Spike S1 protein (active).
There are currently no reviews for SARS-CoV-2 (COVID-19) Spike S1 protein, His and Avi tag (active) (GTX01548-pro). Be the first to share your experience with this product.
