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SARS-CoV-2 (COVID-19) Spike S1 protein, His and Avi tag (active)

SARS-CoV-2 (COVID-19) Spike S1 protein, His and Avi tag (active). GTX01548-pro
SARS-CoV-2 (COVID-19) Spike S1 protein, His and Avi tag (active). GTX01548-pro
SARS-CoV-2 (COVID-19) Spike S1 protein, His and Avi tag (active). GTX01548-pro
SARS-CoV-2 (COVID-19) Spike S1 protein, His and Avi tag (active). GTX01548-pro
SARS-CoV-2 (COVID-19) Spike S1 protein, His and Avi tag (active). GTX01548-pro

Cat. No. GTX01548-pro

Reference 1

Application

 ELISA, Functional Assay

Species

 SARS Coronavirus 2
Package
100 μg ($539)
See all SARS-CoV-2 (COVID-19) Spike products

APPLICATION

Application Note

Activity was measured by its binding ability in a functional ELISA. ImmobilizedSARS-CoV-2 (COVID-19) Spike S1 protein at 0.5 μg/mL (100 μL/well) can bind Recombinant Human ACE2 with a linear range of 0.06-0.25 μg/mL.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with distilled water to 0.1-0.5mg/ml. Lyophilized from 0.22μm-filtered PBS (pH7.4).

Preservative

No preservative

Storage

Store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

Gln14-Arg683 of SARS-CoV-2 (COVID-19) Spike S1 protein (Accession #YP_009724390.1) fused with a 6×His tag and Avi at the C-terminus.

Expression System

HEK293 cells

Purity

> 95% by SDS-PAGE

Endotoxin

< 0.1 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SARS-CoV-2 Spike , COVID-19 Spike

Database

Research Area

DATA IMAGES

SARS-CoV-2 (COVID-19) Spike S1 protein, His and Avi tag (active). GTX01548-pro

GTX01548-pro Functional Assay Image

Inhibition ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His and Avi tag (active) (GTX01548-pro) (coated at 2 μg/mL) inhibiting by SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) (14-10000 ng/mL), and binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (3000 ng/mL). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike S1 protein, His and Avi tag (active). GTX01548-pro

GTX01548-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His and Avi tag (active) protein (GTX01548-pro) (4000-62.5 ng/mL). Coated protein probed with SARS-CoV-2 (COVID-19) Spike antibody (GTX135360) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

SARS-CoV-2 (COVID-19) Spike S1 protein, His and Avi tag (active). GTX01548-pro

GTX01548-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 protein (GTX01548-pro) (62.5-4000 ng/mL). Coated protein probed with SARS-CoV-2 (COVID-19) Spike antibody (GTX135356) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

SARS-CoV-2 (COVID-19) Spike S1 protein, His and Avi tag (active). GTX01548-pro

GTX01548-pro Functional Assay Image

Functional ELISA analysis of immobilized GTX01548-pro SARS-CoV-2 (COVID-19) Spike S1 protein (active) at 0.5μg/mL (100 μL/well) can bind recombinant human ACE2 with a linear range of 0.06-0.25μg/mL.

SARS-CoV-2 (COVID-19) Spike S1 protein, His and Avi tag (active). GTX01548-pro

GTX01548-pro Image

SDS-PAGE of GTX01548-pro SARS-CoV-2 (COVID-19) Spike S1 protein (active).

REFERENCE

Application Reference

Ganneru B. et al. iScience 2021; 24 (4) : 102298 Th1 skewed immune response of whole virion inactivated SARS CoV 2 vaccine and its safety evaluation.
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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 539