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SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448]

Recombinant antibody
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] used in ELISA (ELISA). GTX635686
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] used in ELISA (ELISA). GTX635686
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635686
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] used in Western Blot (WB). GTX635686
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635686
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] used in IHC (Paraffin sections) (IHC-P). GTX635686
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] used in IHC (Paraffin sections) (IHC-P). GTX635686
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] used in ELISA (ELISA). GTX635686
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] used in ELISA (ELISA). GTX635686
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] used in Western Blot (WB). GTX635686
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] used in Western Blot (WB). GTX635686
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] used in ELISA (ELISA). GTX635686
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] used in ELISA (ELISA). GTX635686
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] used in ELISA (ELISA). GTX635686
Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] used in ELISA (ELISA). GTX635686

Cat No. GTX635686

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL448

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA, Lateral Flow, Sandwich ELISA, IHC-P (cell pellet)

Reactivity

SARS Coronavirus, SARS Coronavirus 2
Package
100 μl ($369),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:5000-1:10000
ELISA Assay dependent
Lateral Flow Assay dependent
Sandwich ELISA Assay dependent
IHC-P (cell pellet) Assay dependent

Note :

IHC-P
Recommended heat-Induced Epitope Retrieval pH 6.0 for 20 minutes and background Eraser (BGE) blocking step.

Lateral Flow
This antibody has been validated by lateral flow assay based on the customer’s feedback.

Sandwich ELISA
Capture : GTX635686 , Detection: GTX635678 / GTX635688. or Capture : GTX635685 / GTX635689 / GTX635679 , Detection: GTX635686-01.
Please notice that GTX635688 / GTX635678 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635686. Please contact us for custom HRP-conjugated antibody.

Not tested in other applications.

Product Note

This antibody detects both SARS-CoV nucleocapsid and SARS-CoV-2 nucleocapsid proteins. Our internal testing indicates no cross-reactivity with MERS-CoV nucleocapsid protein.Gold conjugation version is available upon request.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus 2

Immunogen

Full length SARS-CoV-2 (COVID-19) nucleocapsid Recombinant protein. (SARS-CoV-2 (strain Wuhan-Hu-1))

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888557

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

2019-nCoV Nucleocapsid , SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid , 2019-nCoV NP , SARS-CoV-2 NP , COVID-19 NP

Research Area

DATA IMAGES

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] used in ELISA (ELISA). GTX635686

GTX635686 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL249] (GTX635678) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635678 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635686. Please contact us for custom HRP-conjugated antibody.

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] used in ELISA (ELISA). GTX635686

GTX635686 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635688 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635686. Please contact us for custom HRP-conjugated antibody.

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635686

GTX635686 ICC/IF Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] used in Western Blot (WB). GTX635686

GTX635686 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635686

GTX635686 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] used in IHC (Paraffin sections) (IHC-P). GTX635686

GTX635686 IHC-P Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis of SARS-CoV-2 infected human lung.

The IHC-P was performed by HISTOWIZ.

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] used in IHC (Paraffin sections) (IHC-P). GTX635686

GTX635686 IHC-P Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis of SARS-CoV-2 infected human placenta (left) and normal human placenta (right).

The IHC-P was performed by HISTOWIZ.

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] used in ELISA (ELISA). GTX635686

GTX635686 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635686 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635689. Please contact us for custom HRP-conjugated antibody.

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] used in ELISA (ELISA). GTX635686

GTX635686 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635686 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635685. Please contact us for custom HRP-conjugated antibody.

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] used in Western Blot (WB). GTX635686

GTX635686 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] used in Western Blot (WB). GTX635686

GTX635686 WB Image

Non-transfected (–) and transfected (+) Vero E6 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] used in ELISA (ELISA). GTX635686

GTX635686 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (4.8*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
EC50 : 0.011 nM

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] used in ELISA (ELISA). GTX635686

GTX635686 ELISA Image

Sandwich ELISA detection of recombinant full-length Nucleocapsid proteins derived from different strains of SARS-CoV-2 (COVID-19) (Wild type or B.1.617.2 delta variant) using antibodies as below.
Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (1 μg/mL).

Please notice that GTX635686 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635689. Please contact us for custom HRP-conjugated antibody.

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] used in ELISA (ELISA). GTX635686

GTX635686 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135592-pro) (1000-15.625 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] used in ELISA (ELISA). GTX635686

GTX635686 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y, R385K), His Tag (delta variant) and SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (wild type) (GTX135592-pro) (21.91-0.34 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

REFERENCE

There are currently no references for SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686). Be the first to share your publications with this product.

REVIEW

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SDS
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