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SARS-CoV Nucleocapsid antibody [18F629.1]

Anti-SARS-CoV Nucleocapsid antibody [18F629.1] used in Western Blot (WB). GTX30793

Cat No. GTX30793

Host Mouse
Clonality Monoclonal
Clone Name 18F629.1
Isotype IgM
Application WB
Reactivity SARS coronavirus
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
Not tested in other applications.

Predict Reactivity

Mouse, Rat, Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

PBS, 0.2% Gelatin, 0.05% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antibody was developed by immunizing mice with a KLH-conjugated peptide corresponding to amino acids 354-385 of human nucleocapsid protein (Genbank accession no. NP_828858).

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

N antibody N protein antibody N structural protein antibody NC antibody Nucleocapsid protein antibody SARS coronavirus N protein antibody SARS coronavirus nucleocapsid protein antibody SARS CoV antibody SARS CoV N protein antibody SARS CoV nucleocapsid pr

Background

Severe Acute Respiratory Syndrome (SARS), an emerging disease characterized by atypical pneumonia, has recently been attributed to a novel coronavirus (SARS-CoV). SARS is caused by a human coronavirus, which are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics. The nucleocapsid protein of SARS shares little homology with nucleocapsid proteins of other members of the coronavirus family. Nucleocapsid proteins of other coronavirus have been reported to be involved in forming the viral core and also in the packaging and transcription of the viral RNA.

Research Area

DATA IMAGES
Anti-SARS-CoV Nucleocapsid antibody [18F629.1] used in Western Blot (WB). GTX30793

GTX30793 WB Image

WB analysis of (A) mouse melanoma cell transfected with SARS Nucleocapsid or (B) mock control cell lysate using GTX30793 SARS-CoV Nucleocapsid antibody [18F629.1].
Dilution : 1:1000

Package List Price ($)
$ 319