Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with SDF1 antibody [N1C3] (GTX116092) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDF1 antibody [N1C3] detects SDF1 protein by immunofluorescent analysis.
Sample: DIV9 rat cortical neuron and Glia cell cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SDF1 stained by SDF1 antibody [N1C3] (GTX116092) diluted at 1:50.
Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.

Various extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SDF1 antibody [N1C3] (GTX116092) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal Evid Based Complement Alternat Med in 2018.PMID: 29599809