Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1117] (GTX636335) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1117] (GTX636335) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

HepG2 and mitochondria extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1117] (GTX636335) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDHA antibody [HL1117] detects SDHA protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: SDHA stained by SDHA antibody [HL1117] (GTX636335) diluted at 1:500.
Red: MitoTracker® Red CMXRos, a mitochondria marker.
Blue: Fluoroshield with DAPI (GTX30920).

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1117] (GTX636335) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1117] (GTX636335) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1117] (GTX636335) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rat tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1117] (GTX636335) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDHA antibody [HL1117] detects SDHA protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SDHA stained by SDHA antibody [HL1117] (GTX636335) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

SDHA antibody [HL1117] detects SDHA protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatocellular carcinoma.
SDHA stained by SDHA antibody [HL1117] (GTX636335) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDHA antibody [HL1117] detects SDHA protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: SDHA stained by SDHA antibody [HL1117] (GTX636335) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

SDHA antibody [HL1117] detects SDHA protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
SDHA stained by SDHA antibody [HL1117] (GTX636335) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole zebrafish extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [HL1117] (GTX636335) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.