Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SEMA7A antibody [HL1826] (GTX637551) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.