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SHP1 antibody

Anti-SHP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX102864
Anti-SHP1 antibody used in Western Blot (WB). GTX102864
Anti-SHP1 antibody used in Immunoprecipitation (IP). GTX102864
Anti-SHP1 antibody used in Western Blot (WB). GTX102864
Anti-SHP1 antibody used in Western Blot (WB). GTX102864

Cat No. GTX102864

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Raji , NIH-3T3 , rat liver , *U2OS

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.29 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SHP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein tyrosine phosphatase non-receptor type 6 , HCP , HCPH , HPTP1C , PTP-1C , SH-PTP1 , SHP-1 , SHP-1L , SHP1

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SHP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX102864

GTX102864 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using SHP1(GTX102864) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SHP1 antibody used in Western Blot (WB). GTX102864

GTX102864 WB Image

SHP1 antibody detects SHP1 protein by western blot analysis.
A. 50 μg rat liver extract
7.5% SDS-PAGE
SHP1 antibody (GTX102864) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SHP1 antibody used in Immunoprecipitation (IP). GTX102864

GTX102864 IP Image

SHP1 antibody immunoprecipitates SHP1 protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of SHP1 protein expressing HeLa cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of SHP1 by 3 μg of SHP1 antibody (GTX102864) 7.5% SDS-PAGE The immunoprecipitated SHP1 protein was detected by SHP1 antibody (GTX102864) diluted at 1 : 500. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-SHP1 antibody used in Western Blot (WB). GTX102864

GTX102864 WB Image

Sample (30 μg of whole cell lysate)
A:NIH-3T3
7.5% SDS PAGE
GTX102864 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SHP1 antibody used in Western Blot (WB). GTX102864

GTX102864 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SHP1 antibody (GTX102864) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

SHP1 antibody Cat No. GTX102864

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 27-Jan-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 40μg
Blocking : milk, 20°C, 1Hr
Primary Antibody : 1:500, 4°C, 12Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169