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SIGLEC8 antibody [N1N3]

Anti-SIGLEC8 antibody [N1N3] used in Western Blot (WB). GTX117827

Cat No. GTX117827

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

THP-1

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human SIGLEC8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618849

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

sialic acid binding Ig like lectin 8 , SAF2 , SIGLEC-8 , SIGLEC8L

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-SIGLEC8 antibody [N1N3] used in Western Blot (WB). GTX117827

GTX117827 WB Image

Sample (30 ug of whole cell lysate)
A: THP-1
10% SDS PAGE
GTX117827 diluted at 1:1000

REFERENCE

There are currently no references for SIGLEC8 antibody [N1N3] (GTX117827). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for SIGLEC8 antibody [N1N3] (GTX117827). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169