Immunoprecipitation of SIN3B protein from HCT-116 whole cell extracts using 5 μg of SIN3B antibody [N1N2-2], N-term (GTX116417).
Western blot analysis was performed using SIN3B antibody [N1N2-2], N-term (GTX116417) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Sample (50 ug of whole cell lysate)
A: mouse brain
5% SDS PAGE
GTX116417 diluted at 1:500

SIN3B antibody [N1N2-2], N-term detects SIN3B protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SIN3B protein stained by SIN3B antibody [N1N2-2], N-term (GTX116417) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Sample (30 ug of whole cell lysate)
A: HCT116
10% SDS PAGE
GTX116417 diluted at 1:1000