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SIRP alpha antibody [MRC OX-41]

Anti-SIRP alpha antibody [MRC OX-41] used in Flow cytometry (FACS). GTX29295

Cat. No. GTX29295

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MRC OX-41

Isotype

 IgG2a

Application

 WB, IHC-P, IHC-Fr, FACS

Reactivity

 Mouse, Rat
Package
125 μl ($399)
See all SIRP alpha products

APPLICATION

Application Note

Flow Cytometry: 1:100Immunohistochemistry (frozen): 1:20 - 1:100Immunohistochemistry (paraffin): 1:20 - 1:100Flow cytometry: Use 10μl of the suggested working dilution to label 10^6 cells. Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.

Calculated MW

56 kDa. ( Note )

Product Note

This antibody recognises rat SIRP (Signal regulatory protein), which is expressed selectively by myeloid cells and by neurons.

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Rat peritoneal macrophages

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

signal-regulatory protein alpha , Bit , Ptpns1 , SHPS-1

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

plays a role in macrophage fusion [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-SIRP alpha antibody [MRC OX-41] used in Flow cytometry (FACS). GTX29295

GTX29295 FACS Image

Staining of mouse peritoneal macrophages using SIRP antibody (GTX29295) and purified mouse IgG2a isotype control.

REFERENCE

Clone Reference

Lacotte S et al. PLoS One 2014; 9 (3):e91515 Alloimmune activation promotes anti-cancer cytotoxicity after rat liver transplantation.
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399