Flow Cytometry: 1:100Immunohistochemistry (frozen): 1:20 - 1:100Immunohistochemistry (paraffin): 1:20 - 1:100Flow cytometry: Use 10μl of the suggested working dilution to label 10^6 cells. Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
This antibody recognises rat SIRP (Signal regulatory protein), which is expressed selectively by myeloid cells and by neurons.
Ascitic fluid with 0.1% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
8mg/ml(Please refer to the vial label for the specific concentration.)
Rat peritoneal macrophages
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Bit, Ptpns1, SHPS-1, SHPS 1, SHPS1
Protein tyrosine phosphatases (PTPases) SHP-1 andSHP-2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP-2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPa (1), SHPS-1 (2,3), MyD-1 (4), BIT (5,6) and p84 (7). SIRPa is a new gene family containing at least fifteen members. SIRPa is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP-2 (1,2,5,8). SIRPa has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion and plays a general role in different physiological and pathological processes (1,2,5,8).