Wild-type (WT) and SIRT1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SIRT1 antibody (GTX134606) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SIRT1 antibody (GTX134606) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

SIRT1 antibody detects SIRT1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SIRT1 stained by SIRT1 antibody (GTX134606) diluted at 1:500.

SIRT1 antibody detects SIRT1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
SIRT1 stained by SIRT1 antibody (GTX134606) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min