Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SMC1 (phospho Ser957) antibody [GT1321] (GTX03233) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

WB analysis of NIH-3T3 cells were treated by UV at room temperature for 15-30 minutes using GTX03233 SMC1 (phospho Ser957) antibody [GT1321].
Dilution : 1:1000
Loading : 25μg per lane

WB analysis of 293T cells were treated by UV at room temperature for 15-30 minutes using GTX03233 SMC1 (phospho Ser957) antibody [GT1321].
Dilution : 1:1000
Loading : 25μg per lane

IHC-P analysis of mouse testis tissue section using GTX03233 SMC1 (phospho Ser957) antibody [GT1321].
Dilution : 1:100

IHC-P analysis of human colon tissue section using GTX03233 SMC1 (phospho Ser957) antibody [GT1321].
Dilution : 1:100

IHC-P analysis of rat brain tissue section using GTX03233 SMC1 (phospho Ser957) antibody [GT1321].
Dilution : 1:100