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SMC1 (phospho Ser957) antibody [GT1321]

Recombinant antibody Orthogonal
Anti-SMC1 (phospho Ser957) antibody [GT1321] used in Western Blot (WB). GTX03233
Anti-SMC1 (phospho Ser957) antibody [GT1321] used in IHC (Paraffin sections) (IHC-P). GTX03233
Anti-SMC1 (phospho Ser957) antibody [GT1321] used in IHC (Paraffin sections) (IHC-P). GTX03233
Anti-SMC1 (phospho Ser957) antibody [GT1321] used in IHC (Paraffin sections) (IHC-P). GTX03233
Anti-SMC1 (phospho Ser957) antibody [GT1321] used in Western Blot (WB). GTX03233
Anti-SMC1 (phospho Ser957) antibody [GT1321] used in Western Blot (WB). GTX03233

Cat. No. GTX03233

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 GT1321

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419)
See all SMC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

143 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.61 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S957 of human SMC1.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

structural maintenance of chromosomes 1A , CDLS2 , DXS423E , SB1.8 , SMC1 , SMC1L1 , SMC1alpha , SMCB

Cellular Localization

Nucleus

Background

Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in this gene result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-SMC1 (phospho Ser957) antibody [GT1321] used in Western Blot (WB). GTX03233

GTX03233 WB Image

Orthogonal

Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SMC1 (phospho Ser957) antibody [GT1321] (GTX03233) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SMC1 (phospho Ser957) antibody [GT1321] used in IHC (Paraffin sections) (IHC-P). GTX03233

GTX03233 IHC-P Image

IHC-P analysis of rat brain tissue section using GTX03233 SMC1 (phospho Ser957) antibody [GT1321].
Dilution : 1:100

Anti-SMC1 (phospho Ser957) antibody [GT1321] used in IHC (Paraffin sections) (IHC-P). GTX03233

GTX03233 IHC-P Image

IHC-P analysis of human colon tissue section using GTX03233 SMC1 (phospho Ser957) antibody [GT1321].
Dilution : 1:100

Anti-SMC1 (phospho Ser957) antibody [GT1321] used in IHC (Paraffin sections) (IHC-P). GTX03233

GTX03233 IHC-P Image

IHC-P analysis of mouse testis tissue section using GTX03233 SMC1 (phospho Ser957) antibody [GT1321].
Dilution : 1:100

Anti-SMC1 (phospho Ser957) antibody [GT1321] used in Western Blot (WB). GTX03233

GTX03233 WB Image

Orthogonal

WB analysis of 293T cells were treated by UV at room temperature for 15-30 minutes using GTX03233 SMC1 (phospho Ser957) antibody [GT1321].
Dilution : 1:1000
Loading : 25μg per lane

Anti-SMC1 (phospho Ser957) antibody [GT1321] used in Western Blot (WB). GTX03233

GTX03233 WB Image

Orthogonal

WB analysis of NIH-3T3 cells were treated by UV at room temperature for 15-30 minutes using GTX03233 SMC1 (phospho Ser957) antibody [GT1321].
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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Package List Price ($)
$ 419