APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS pH7.3, 0.05% BSA, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.61 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A phospho specific peptide corresponding to residues surrounding S957 of human SMC1.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
structural maintenance of chromosomes 1A , CDLS2 , DXS423E , SB1.8 , SMC1 , SMC1L1 , SMC1alpha , SMCB
Cellular Localization
Nucleus
Background
Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in this gene result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]
Database
Research Area
DATA IMAGES
![Anti-SMC1 (phospho Ser957) antibody [GT1321] used in Western Blot (WB). GTX03233](/templates/default/js/lightbox/images/loading.gif "Anti-SMC1 (phospho Ser957) antibody [GT1321] used in Western Blot (WB). GTX03233")
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GTX03233 WB Image
Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SMC1 (phospho Ser957) antibody [GT1321] (GTX03233) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX03233 IHC-P Image
IHC-P analysis of mouse testis tissue section using GTX03233 SMC1 (phospho Ser957) antibody [GT1321].
Dilution : 1:100
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GTX03233 IHC-P Image
IHC-P analysis of rat brain tissue section using GTX03233 SMC1 (phospho Ser957) antibody [GT1321].
Dilution : 1:100
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GTX03233 IHC-P Image
IHC-P analysis of human colon tissue section using GTX03233 SMC1 (phospho Ser957) antibody [GT1321].
Dilution : 1:100
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GTX03233 WB Image
WB analysis of NIH-3T3 cells were treated by UV at room temperature for 15-30 minutes using GTX03233 SMC1 (phospho Ser957) antibody [GT1321].
Dilution : 1:1000
Loading : 25μg per lane
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GTX03233 WB Image
WB analysis of 293T cells were treated by UV at room temperature for 15-30 minutes using GTX03233 SMC1 (phospho Ser957) antibody [GT1321].
Dilution : 1:1000
Loading : 25μg per lane
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REFERENCE
There are currently no references for SMC1 (phospho Ser957) antibody [GT1321] (GTX03233). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for SMC1 (phospho Ser957) antibody [GT1321] (GTX03233). Be the first to share your experience with this product.
![Anti-SMC1 (phospho Ser957) antibody [GT1321] used in Western Blot (WB). GTX03233](/upload/website/prouct_img/small/GTX03233/GTX03233_4000001606_20210716_WB_CIP_21072300_347.jpg "Anti-SMC1 (phospho Ser957) antibody [GT1321] used in Western Blot (WB). GTX03233")
![Anti-SMC1 (phospho Ser957) antibody [GT1321] used in IHC (Paraffin sections) (IHC-P). GTX03233](/upload/website/prouct_img/small/GTX03233/GTX03233_20210615_IHC-P_94_21061419_793.jpg "Anti-SMC1 (phospho Ser957) antibody [GT1321] used in IHC (Paraffin sections) (IHC-P). GTX03233")
![Anti-SMC1 (phospho Ser957) antibody [GT1321] used in IHC (Paraffin sections) (IHC-P). GTX03233](/upload/website/prouct_img/small/GTX03233/GTX03233_20210615_IHC-P_92_21061419_647.jpg "Anti-SMC1 (phospho Ser957) antibody [GT1321] used in IHC (Paraffin sections) (IHC-P). GTX03233")
![Anti-SMC1 (phospho Ser957) antibody [GT1321] used in IHC (Paraffin sections) (IHC-P). GTX03233](/upload/website/prouct_img/small/GTX03233/GTX03233_20210615_IHC-P_93_21061419_774.jpg "Anti-SMC1 (phospho Ser957) antibody [GT1321] used in IHC (Paraffin sections) (IHC-P). GTX03233")
![Anti-SMC1 (phospho Ser957) antibody [GT1321] used in Western Blot (WB). GTX03233](/upload/website/prouct_img/small/GTX03233/GTX03233_98_WB_21080201_758.jpg "Anti-SMC1 (phospho Ser957) antibody [GT1321] used in Western Blot (WB). GTX03233")
![Anti-SMC1 (phospho Ser957) antibody [GT1321] used in Western Blot (WB). GTX03233](/upload/website/prouct_img/small/GTX03233/GTX03233_97_WB_21080201_292.jpg "Anti-SMC1 (phospho Ser957) antibody [GT1321] used in Western Blot (WB). GTX03233")
![Anti-SMC1 (phospho Ser957) antibody [GT1321] used in Western Blot (WB). GTX03233](/upload/website/prouct_img/normal/GTX03233/GTX03233_4000001606_20210716_WB_CIP_21072300_347.jpg "Anti-SMC1 (phospho Ser957) antibody [GT1321] used in Western Blot (WB). GTX03233")
![Anti-SMC1 (phospho Ser957) antibody [GT1321] used in IHC (Paraffin sections) (IHC-P). GTX03233](/upload/website/prouct_img/normal/GTX03233/GTX03233_20210615_IHC-P_94_21061419_793.jpg "Anti-SMC1 (phospho Ser957) antibody [GT1321] used in IHC (Paraffin sections) (IHC-P). GTX03233")
![Anti-SMC1 (phospho Ser957) antibody [GT1321] used in IHC (Paraffin sections) (IHC-P). GTX03233](/upload/website/prouct_img/normal/GTX03233/GTX03233_20210615_IHC-P_92_21061419_647.jpg "Anti-SMC1 (phospho Ser957) antibody [GT1321] used in IHC (Paraffin sections) (IHC-P). GTX03233")
![Anti-SMC1 (phospho Ser957) antibody [GT1321] used in IHC (Paraffin sections) (IHC-P). GTX03233](/upload/website/prouct_img/normal/GTX03233/GTX03233_20210615_IHC-P_93_21061419_774.jpg "Anti-SMC1 (phospho Ser957) antibody [GT1321] used in IHC (Paraffin sections) (IHC-P). GTX03233")
![Anti-SMC1 (phospho Ser957) antibody [GT1321] used in Western Blot (WB). GTX03233](/upload/website/prouct_img/normal/GTX03233/GTX03233_98_WB_21080201_758.jpg "Anti-SMC1 (phospho Ser957) antibody [GT1321] used in Western Blot (WB). GTX03233")
![Anti-SMC1 (phospho Ser957) antibody [GT1321] used in Western Blot (WB). GTX03233](/upload/website/prouct_img/normal/GTX03233/GTX03233_97_WB_21080201_292.jpg "Anti-SMC1 (phospho Ser957) antibody [GT1321] used in Western Blot (WB). GTX03233")