Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SMN1 antibody (GTX101047) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed HeLa, using SMN1(GTX101047) antibody at 1:200 dilution.