GeneTex
United States (US)

SNAI1 antibody

Comparison Orthogonal Overexpression
GTX100754_42221_20171215_WB_treat_TGF-beta_EGF_competitor_watermark_19011506_390.jpg
GTX100754_42221_20190111_WB_B_19021719_447.jpg
GTX100754_42221_20150903_WB_19011506_877.jpg
GTX100754_42221_20161027_WB_TGF-beta_EGF_19011506_152.jpg
GTX100754_42221_20161027_IFA_19011506_317.jpg

Cat No. GTX100754

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS , HeLa , Hela (1 ng/ml TGF-beta and 300 ng/ml of EGF for 2 hr) , HeLa (starvation for 16 hr, 20 ng/ml TGF-beta and 300 ng/ml EGF treatment for 30 min)

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.96mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SNAI1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Snail Family Transcriptional Repressor 1,Slugh2,Sna,Snah,Snail,Snail1,Dj710H13.1,Snai1

Cellular Localization

Nucleus

Background

The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100754_42221_20171215_WB_treat_TGF-beta_EGF_competitor_watermark_19011506_390.jpg

GTX100754 WB Image

Comparison Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (50 µg) were separated by 10% SDS-PAGE, and the membranes were blotted with SNAI1 antibody (GTX100754) diluted at 1:1000 and competitor's antibody (#3879) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX100754_42221_20190111_WB_B_19021719_447.jpg

GTX100754 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody (GTX100754) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100754_42221_20150903_WB_19011506_877.jpg

GTX100754 WB Image

SNAI1 antibody detects SNAI1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody (GTX100754) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100754_42221_20161027_WB_TGF-beta_EGF_19011506_152.jpg

GTX100754 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody (GTX100754) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100754_42221_20161027_IFA_19011506_317.jpg

GTX100754 ICC/IF Image

SNAI1 antibody detects SNAI1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SNAI1 protein stained by SNAI1 antibody (GTX100754) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE
REVIEW

SNAI1 antibody Cat No. GTX100754

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 03-Oct-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : TW2.6 cells
Amount used : 15μg
Blocking : 5% milk in PBST, 25°C, 1Hr
Primary Antibody : 1:3000, 25°C, 1Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169