APPLICATION
Application Note
ELISA: Use at an assay dependent dilution. IHC: Use at a concentration of 1 μg/ml. IP: Use at a concentration of 10 μg/ml. WB: Use at a concentration of 0.2 μg/ml. Detects a band of approximately 19 and 23 kDa (predicted molecular weight: 18 kDa). Optimal dilutions/concentrations should be determined by the end user.
Calculated MW
Positive Control
HeLa Cell Lysate
PROPERTIES
Form
Liquid
Buffer
0.1M Sodium Phosphate pH7.0, 50% Glycerol
Preservative
0.1% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length protein (Human).
Purification
Immunogen affinity purified
This antibody was purified on an antigen coupled sepharose column.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
superoxide dismutase 1 , ALS , ALS1 , HEL-S-44 , IPOA , SOD , hSod1 , homodimer
Cellular Localization
Cytoplasm , Mitochondrion , Nucleus
Background
The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX13498 IHC Image
Immunohistochemistry analysis of human heart tissue stained with GTX13498 at 10 ug/mL.
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GTX13498 WB Image
Western blot analysis of GTX13498.
Lane 1: Rat liver tissue lysate
Lane 2: Mouse liver tissue lysate
Lane 3: HeLa cell lysate.
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GTX13498 ICC/IF Image
Immunofluorescent analysis (confocal) staining of HeLa cells using GTX13498 (green); nuclei are stained in blue pseudo-color using DRAQ5.
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REFERENCE
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REVIEW
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