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SOX10 antibody [SOX10/1074]

Anti-SOX10 antibody [SOX10/1074] used in Western Blot (WB). GTX35084
Anti-SOX10 antibody [SOX10/1074] used in IHC (Paraffin sections) (IHC-P). GTX35084
Anti-SOX10 antibody [SOX10/1074] used in IHC (Paraffin sections) (IHC-P). GTX35084

Cat No. GTX35084

Host Mouse
Clonality Monoclonal
Clone Name SOX10/1074
Isotype IgG2b
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1.0ug/ml
ICC/IF 1-2ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Specificity/Sensitivity

Recognizes a protein of ~55kDa, identified as SOX10. This MAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately to strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-10 expression is also observed in mast cells.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human SOX10 protein fragment (aa115-269) (exact sequence is proprietary)

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Sry-Box 10,Dom,Pcwh,Ws2E,Ws4,Ws4C,Sox10

Background

This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-SOX10 antibody [SOX10/1074] used in Western Blot (WB). GTX35084

GTX35084 WB Image

Western blot analysis of SOX10 (A) Recombinant protein; (B) A375 Cell lysate using SOX10 MAb (SOX10/1074).

Anti-SOX10 antibody [SOX10/1074] used in IHC (Paraffin sections) (IHC-P). GTX35084

GTX35084 IHC-P Image

Formalin-fixed, paraffin-embedded Human Melanoma stained with SOX10 MAb (SOX10/1074).

Anti-SOX10 antibody [SOX10/1074] used in IHC (Paraffin sections) (IHC-P). GTX35084

GTX35084 IHC-P Image

Formalin-fixed, paraffin-embedded Mouse Brain SOX10 MAb (SOX10/1074).

Package List Price ($)
$ 329