SOX2 antibody [HL1192] detects SOX2 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 1 day-post-fertilization zebrafish embryo.
Green: SOX2 stained by SOX2 antibody [HL1192] (GTX636504) diluted at 1:100.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Zebrafish tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [HL1192] (GTX636504) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.