Summary
SOX2 antibody detects SOX2 protein, a basic helix-loop-helix transcription factor (predicted molecular weight of 34 kDa). SOX2 is an indispensable factor for regulating embryonic development and in the generation of inducible pluripotent stem (iPS) cells. It is also expressed in neural stem and progenitor cells. Dysregulation of SOX2 is associated with tumorigenesis.
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:5000-1:20000 |
1:100-1:1000 |
1:100-1:1000 |
1:100-1:1000 |
1:50-1:200 |
1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
NT2D1 , hESC , Mouse ESC
Predict Reactivity
Zebrafish, Sheep, Bovine, Cat, Chicken, Pig, Japanese Medaka, Xenopus laevis, Rhesus Monkey(>80% identity)
Form
Liquid
Buffer
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.15 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human SOX2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2038021
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Synonyms
SRY-box 2 , ANOP3 , MCOPS3
Cellular Localization
Nucleus
Background
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq]
Database
Research Area