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SPARC antibody

Anti-SPARC antibody used in IHC (Paraffin sections) (IHC-P). GTX55805
Anti-SPARC antibody used in IHC (Paraffin sections) (IHC-P). GTX55805
Anti-SPARC antibody used in Western Blot (WB). GTX55805
Anti-SPARC antibody used in IHC (Paraffin sections) (IHC-P). GTX55805

Cat. No. GTX55805

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all SPARC products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 18-303 of human SPARC (NP_003109.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

secreted protein acidic and cysteine rich , BM-40 , OI17 , ON

Background

This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015]

Database

Research Area

DATA IMAGES

Anti-SPARC antibody used in IHC (Paraffin sections) (IHC-P). GTX55805

GTX55805 IHC-P Image

IHC-P analysis of rat intestine tissue using GTX55805 SPARC antibody.
Dilution : 1:100

Anti-SPARC antibody used in IHC (Paraffin sections) (IHC-P). GTX55805

GTX55805 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX55805 SPARC antibody.
Dilution : 1:100

Anti-SPARC antibody used in Western Blot (WB). GTX55805

GTX55805 WB Image

WB analysis of various sample lysates using GTX55805 SPARC antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-SPARC antibody used in IHC (Paraffin sections) (IHC-P). GTX55805

GTX55805 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX55805 SPARC antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399