WB analysis of various samples using GTX20265 SPRY4 antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : SW13 whole cell lysate
Lane 3 : C2C12 whole cell lysate
Loading : 30 μg
Dilution : 1:100

IHC-P analysis of human heart tissue using GTX20265 SPRY4 antibody.
Dilution : 2.5 μg/mL

IHC-P analysis of human liver tissue using GTX20265 SPRY4 antibody.
Dilution : 2.5 μg/mL

Western Blot of Rabbit anti-Sprouty-4 antibody. Lane 1: HeLa. Lane 2: SW13. Lane 3: C2C12. Load: 30 μg per lane.Primary antibody: Sprouty 4 antibody at 1:100 for overnight at 4ºC.Secondary antibody: IRDye800™ rabbit secondary antibody at 1:5,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4ºC.Predicted/Observed size: a doublet band ~35 kDa corresponding to human Sprouty-4. Other band(s): none.

Immunohistochemistry of Rabbit anti-Sprouty-4 antibody. Tissue: human liver tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: Sprouty-4 antibody at 2.5 μg/mL for 1 h at RT.Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.Localization: Sprouty-4 is cytoplasmic.Staining: Sprouty-4 as precipitated brown with blue nuclear counterstain.

Immunohistochemistry of Rabbit anti-Sprouty-4 antibody. Tissue: human heart tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: Sprouty-4 antibody at 2.5 μg/ml for 1 h at RT.Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.Localization: Sprouty-4 is cytoplasmic.Staining: Sprouty-4 as precipitated red signal with hematoxylin purple nuclear counterstain.