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SQSTM1 / P62 antibody [GT1478]

KO/KD Comparison Orthogonal
Anti-SQSTM1 / P62 antibody [GT1478] used in Western Blot (WB). GTX629890
Anti-SQSTM1 / P62 antibody [GT1478] used in Western Blot (WB). GTX629890
Anti-SQSTM1 / P62 antibody [GT1478] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629890
Anti-SQSTM1 / P62 antibody [GT1478] used in IHC (Frozen sections) (IHC-Fr). GTX629890
Anti-SQSTM1 / P62 antibody [GT1478] used in Western Blot (WB). GTX629890
Anti-SQSTM1 / P62 antibody [GT1478] used in Flow cytometry (FACS). GTX629890
Anti-SQSTM1 / P62 antibody [GT1478] used in Western Blot (WB). GTX629890
Anti-SQSTM1 / P62 antibody [GT1478] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629890

Cat. No. GTX629890

Reference 6
9

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1478

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-Fr, FACS

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SQSTM1 / P62 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
FACS 1:50-1:200
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

Huh-7 (3 μM Thapsigargin treatment for 12 hr) , Huh-7 (3 μM Thapsigargin treatment for 24 hr) , HepG2 (3 μM Thapsigargin treatment for 12 hr) , HepG2 (3 μM Thapsigargin treatment for 24 hr) , HepG2

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human SQSTM1 / P62. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2885144

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

sequestosome 1 , A170 , DMRV , FTDALS3 , NADGP , OSIL , PDB3 , ZIP3 , p60 , p62 , p62B

Cellular Localization

Cytoplasm , Late endosome , Nucleus

Background

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SQSTM1 / P62 antibody [GT1478] used in Western Blot (WB). GTX629890

GTX629890 WB Image

Orthogonal

SQSTM1 antibody detects SQSTM1 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12 hr and 24hr) Huh-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody (GTX629890) diluted by 1:1000.
The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SQSTM1 / P62 antibody [GT1478] used in Western Blot (WB). GTX629890

GTX629890 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [GT1478] (GTX629890) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SQSTM1 / P62 antibody [GT1478] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629890

GTX629890 ICC/IF Image

SQSTM1 antibody [GT1478] detects SQSTM1 protein at autophagosome by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SQSTM1 protein stained by SQSTM1 antibody [GT1478] (GTX629890) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.
Anti-SQSTM1 / P62 antibody [GT1478] used in IHC (Frozen sections) (IHC-Fr). GTX629890

GTX629890 IHC-Fr Image

SQSTM1 antibody [GT1478] detects SQSTM1 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: SQSTM1 protein stained by SQSTM1 antibody [GT1478] (GTX629890) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-SQSTM1 / P62 antibody [GT1478] used in Western Blot (WB). GTX629890

GTX629890 WB Image

KO/KD

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody [GT1478] (GTX629890) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SQSTM1 / P62 antibody [GT1478] used in Flow cytometry (FACS). GTX629890

GTX629890 FACS Image

SQSTM1 antibody [GT1478] (GTX629890) detects SQSTM1 protein by flow cytometry analysis.
Sample: HeLa cell fixed in 4% paraformaldehyde at 4ºC for 5 min.
Brown: Unlabelled sample was also used as a control.
Blue: SQSTM1 antibody [GT1478] (GTX629890) dilution: 1:100.
Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-SQSTM1 / P62 antibody [GT1478] used in Western Blot (WB). GTX629890

GTX629890 WB Image

Orthogonal

SQSTM1 antibody detects SQSTM1 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12 hr and 24hr) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody (GTX629890) diluted by 1:1000.
The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-SQSTM1 / P62 antibody [GT1478] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629890

GTX629890 ICC/IF Image

Orthogonal

SQSTM1 / P62 antibody [GT1478] detects SQSTM1 / P62 protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and treated HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: SQSTM1 / P62 stained by SQSTM1 / P62 antibody [GT1478] (GTX629890) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

REFERENCE

Application Reference

Wataru Otsu et al. Sci Rep 2021; 11 (1) : 18555 Cigarette smoke extract and heated tobacco products promote ferritin cleavage and iron accumulation in human corneal epithelial cells.
Application : WB, ICC/IF
Reactivity : Human
Beak JY et al. J Biol Chem 2021; 297 (6) : 101358 The nuclear receptor RORα preserves cardiomyocyte mitochondrial function by regulating caveolin-3-mediated mitophagy.
Application : WB
Reactivity : Rat
Liang YC et al. Cancers (Basel) 2020; 12 (6) The Antihistamine Deptropine Induces Hepatoma Cell Death through Blocking Autophagosome-Lysosome Fusion.
Kiren Baines et al. bioRxiv 2019; (Epub) The ATG5 Interactome Links Clathrin Vesicular Trafficking With The ATG8 Lipidation Machinery For Autophagosome Assembly.
Saito Y et al. Redox Biol 2018; 19 : 354-363 Transient * of autophagic degradation by pharmacological Nrf2 activation is important for retinal pigment epithelium cell survival.
Application : WB
Reactivity : Human
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REVIEW

SQSTM1 / P62 antibody [GT1478] Cat. No. GTX629890

Rating
( Average 4.7 based on 9 users reviews)
Application
Western Blot(WB)
( Average 4.6 based on 8 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-SQSTM1 / P62 antibody [GT1478] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629890
Date : Anonymous submitted on 26-Jul-2022
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : U2OS WT and SQSTM1 KO cells
Blocking : 5% BSA, 5% goat serum and 0.01% Triton X-100 in PBS, 30min, RT
Fixation : 4% PFA in PBS, 15min, RT
Permeabilization /Antigen retrieval : 0.1% Triton X-100 in PBS, 10min, RT
Primary Antibody : 1:1000, 4°C, > 12Hr
Lane Description :
WT cells: Yellow dashed line
KO cells: Magenta dashed line
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-SQSTM1 / P62 antibody [GT1478] used in Western Blot (WB). GTX629890
Date : Anonymous submitted on 26-Jul-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS WT and SQSTM1 KO cells
Amount used : 25μg
Blocking : 5% milk, 1 hr
Primary Antibody : 1:1000 in 5% BSA and TBST, 4°C, > 12Hr
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-SQSTM1 / P62 antibody [GT1478] used in Western Blot (WB). GTX629890
Date : Anonymous submitted on 20-May-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : HCE-T cells
Amount used : 2 μg
Blocking : Blocking One-P, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 18Hr
Anti-SQSTM1 / P62 antibody [GT1478] used in Western Blot (WB). GTX629890
Date : Anonymous submitted on 06-May-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 10 μg
Blocking : 5% skim milk in TBST, 27°C, 1Hr
Primary Antibody : 1:5000, 4°C, 12Hr
Lane Description : Two groups (mock and Dengue virus infection) of whole cell extracts (10 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody (GTX629890) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Anti-SQSTM1 / P62 antibody [GT1478] used in Western Blot (WB). GTX629890
Date : Anonymous submitted on 24-May-2018
Score :
Application Tested : WB
Sample Species : Ms
Sample : Raw264.7 cells
Amount used : 50 μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 37°C, 1Hr
Anti-SQSTM1 / P62 antibody [GT1478] used in Western Blot (WB). GTX629890
Date : Anonymous submitted on 12-May-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : HepG2 cells
Amount used : 30μg
Blocking : 3% BSA in PBST, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 16Hr
Anti-SQSTM1 / P62 antibody [GT1478] used in Western Blot (WB). GTX629890
Date : Anonymous submitted on 24-Apr-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : MNT-1 cells
Amount used : 30μg
Blocking : 5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-SQSTM1 / P62 antibody [GT1478] used in Western Blot (WB). GTX629890
Date : Anonymous submitted on 18-Mar-2015
Score :
Application Tested : WB
Sample Species : Ms
Sample : MEF cells
Amount used : 50μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 14Hr
Anti-SQSTM1 / P62 antibody [GT1478] used in Western Blot (WB). GTX629890
Date : Anonymous submitted on 17-Mar-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : 2C4 cells
Amount used : 50μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 14Hr
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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations