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SQSTM1 / P62 antibody [N3C1], Internal

KO/KD Comparison Orthogonal
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100685
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX100685
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Immunoprecipitation (IP). GTX100685
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Flow cytometry (FACS). GTX100685
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100685
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685

Cat. No. GTX100685

Reference 91
5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, IP, PLA

Reactivity

 Human, Mouse, Rat, Zebrafish, Bovine, Honeybee, Mosquito
Package
100 μl ($399),
25 μl ($169)
See all SQSTM1 / P62 products
PRODUCT

Summary

SQSTM1 antibody, also known as ubiquitin-binding protein p62 antibody, detects the SQSTM1 protein that interacts with LC3 proteins to execute autophagosomal degradation. SQSTM1 is also reported to regulate the activation of NFκB1 downstream signaling in addition to having other cellular functions.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS 1:50-1:200
IP 1:100-1:500
PLA Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

NIH-3T3 , JC , BCL-1 , PC-12 , Rat2 , Huh7 ( 3μM Thapsigargin 12hr) , HepG2

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human SQSTM1 / P62. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038029

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sequestosome 1 , A170 , DMRV , FTDALS3 , NADGP , OSIL , PDB3 , ZIP3 , p60 , p62 , p62B

Cellular Localization

Cytoplasm , Late endosome , Nucleus

Background

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100685

GTX100685 ICC/IF Image

Orthogonal

SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SQSTM1 protein stained by SQSTM1 antibody [N3C1], Internal (GTX100685) diluted at 1:1000.
Red: Phalloidin, a F-actin marker.
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685

GTX100685 WB Image

KO/KD

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody [N3C1], Internal (GTX100685) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685

GTX100685 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [N3C1], Internal (GTX100685) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685

GTX100685 WB Image

Orthogonal

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [N3C1], Internal (GTX100685) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685

GTX100685 WB Image

Orthogonal

Untreated (–) and treated (+) Huh-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody [N3C1], Internal (GTX100685) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685

GTX100685 WB Image

SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
10% SDS-PAGE
SQSTM1 antibody [N3C1], Internal (GTX100685) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685

GTX100685 WB Image

SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein by western blot analysis.
A. 30 μg NIH-3T3 whole cell lysate/extract
B. 30 μg JC whole cell lysate/extract
C. 30 μg BCL-1 whole cell lysate/extract
12% SDS-PAGE
SQSTM1 antibody [N3C1], Internal (GTX100685) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SQSTM1 / P62 antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX100685

GTX100685 IHC-P Image

SQSTM1 / P62 antibody [N3C1], Internal detects SQSTM1 / P62 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
SQSTM1 / P62 stained by SQSTM1 / P62 antibody [N3C1], Internal (GTX100685) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685

GTX100685 WB Image

Orthogonal

Untreated (–) and treated (+) NIH-3T3 whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [N3C1], Internal (GTX100685) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Immunoprecipitation (IP). GTX100685

GTX100685 IP Image

Immunoprecipitation of SQSTM1 protein from HeLa whole cell extracts using 5 μg of SQSTM1 antibody [N3C1], Internal (GTX100685).
Western blot analysis was performed using SQSTM1 antibody [N3C1], Internal (GTX100685).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Flow cytometry (FACS). GTX100685

GTX100685 FACS Image

SQSTM1 antibody [N3C1], Internal (GTX100685) detects SQSTM1 protein by flow cytometry analysis.
Sample: HeLa cell fixed in 4% paraformaldehyde at 4oC for 5 min.
Brown: Unlabelled sample was also used as a control.
Blue: SQSTM1 antibody [N3C1], Internal] (GTX100685) dilution: 1:100.
Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100685

GTX100685 ICC/IF Image

Orthogonal

SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome by immunofluorescent analysis.
Samples: HepG2 cells treated with 3μM thapsigargin 12 hrs (rigtht) and mock (left) were fixed in ice-cold MeOH for 10 min, permeabilize with cooled acetone for 1 min .
Green: SQSTM1 protein stained by SQSTM1 antibody [N3C1], Internal (GTX100685) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685

GTX100685 WB Image

Orthogonal

The data was published in the journal Front Pharmacol in 2017.PMID: 29326587

Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685

GTX100685 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2017. PMID: 28134285

Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685

GTX100685 WB Image

Orthogonal

The data was published in the journal Biomolecules in 2019.PMID: 31505885

Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685

GTX100685 WB Image

Orthogonal

The data was published in the journal Biomolecules in 2019.PMID: 31505885

Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685

GTX100685 WB Image

Orthogonal

The data was published in the journal Antioxidants (Basel) in 2019.PMID: 31906147

Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685

GTX100685 WB Image

Orthogonal

The data was published in the journal Antioxidants (Basel) in 2019.PMID: 31906147

Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685

GTX100685 WB Image

Orthogonal

The data was published in the 2020 in Int J Mol Sci. PMID: 33260618

Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685

GTX100685 WB Image

The data was published in the journal PLoS One in 2015.PMID: 25946033

Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685

GTX100685 WB Image

The data was published in the journal Biomolecules in 2019.PMID: 31505885

REFERENCE

Application Reference

Ma X et al. Heliyon 2024; 10 (19) : e38026 Resistin alleviates lipopolysaccharide-induced inflammation in bovine alveolar macrophages by activating the AMPK/mTOR signaling pathway and autophagy.
X Ma et al. Heliyon 2024; 10 (19) : e38026 Resistin alleviates lipopolysaccharide-induced inflammation in bovine alveolar macrophages by activating the AMPK/mTOR signaling pathway and autophagy
Sini P et al. Front Immunol 2024; 15 : 1360068 Evaluation of cyanotoxin L-BMAA effect on α-synuclein and TDP43 proteinopathy.
YS Lai et al. Open Biol 2024; 14 (4) : 240001 Optogenetically engineered calcium oscillations promote autophagy-mediated cell death via AMPK activation
Application : WB
MJ Young et al. Cell Death Differ 2024; USP24-i-101 targeting of USP24 activates autophagy to inhibit drug resistance acquired during cancer therapy
Application : WB
Show More +
Submit a Reference

REVIEW

SQSTM1 / P62 antibody [N3C1], Internal Cat. No. GTX100685

Rating
( Average 4.4 based on 5 users reviews)
Application
Western Blot(WB)
( Average 4.5 based on 4 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 4 based on 1 users reviews)
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685
Date : Anonymous submitted on 10-May-2024
Score :
Application Tested : WB
Sample Species : Hu
Sample : HK-2 cells
Amount used : 15μg
Blocking : 5% BSA in TBST, 4°C, overnightHr
Primary Antibody : 1:1000, 25°C, 1Hr
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685
Date : Anonymous submitted on 26-Jul-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS WT and SQSTM1 KO cells
Amount used : 25μg
Blocking : 5% milk, 1 hr
Primary Antibody : 1:1000 in 5% BSA and TBST, 4°C, > 12Hr
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX100685
Date : Anonymous submitted on 08-Mar-2022
Score :
Application Tested : IHC-P
Sample Species : Rat
Sample : Rat eye tissue section
Blocking : 5% goat serum, RT, 1Hr
Fixation : PFA
Permeabilization /Antigen retrieval : TBS-T / None
Primary Antibody : 1:100, 4°C, 12Hr
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685
Date : Anonymous submitted on 17-Mar-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 50μg
Blocking : 5% non-fat milk, 4°C, 14Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685
Date : Anonymous submitted on 26-Jan-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 30μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
Application Reference
Ma X et al. Heliyon 2024; 10 (19):e38026 Resistin alleviates lipopolysaccharide-induced inflammation in bovine alveolar macrophages by activating the AMPK/mTOR signaling pathway and autophagy.
X Ma et al. Heliyon 2024; 10 (19):e38026 Resistin alleviates lipopolysaccharide-induced inflammation in bovine alveolar macrophages by activating the AMPK/mTOR signaling pathway and autophagy
Sini P et al. Front Immunol 2024; 15:1360068 Evaluation of cyanotoxin L-BMAA effect on α-synuclein and TDP43 proteinopathy.
YS Lai et al. Open Biol 2024; 14 (4):240001 Optogenetically engineered calcium oscillations promote autophagy-mediated cell death via AMPK activation
Application : WB
MJ Young et al. Cell Death Differ 2024; USP24-i-101 targeting of USP24 activates autophagy to inhibit drug resistance acquired during cancer therapy
Application : WB
Ayoubi R et al. F1000Res 2023; 12:324 The identification of high-performing antibodies for Sequestosome-1 for use in Western blot, immunoprecipitation and immunofluorescence.
Wang SA et al. Mol Oncol 2023; NCI677397 targeting USP24-mediated induction of lipid peroxidation induces ferroptosis in drug-resistant cancer cells
Application : WB
Hong X et al. J Biol Chem 2023; 299 (6):104814 Diarylheptanoid 35d overcomes EGFR TKI resistance by inducing hsp70-mediated lysosomal degradation of EGFR in EGFR-mutant lung adenocarcinoma.
Pan M et al. Autophagy 2023; 19 (2):440-456 UXT attenuates the CGAS-STING1 signaling by targeting STING1 for autophagic degradation.
2023; MDM2 upregulation induces mitophagy deficiency via Mic60 ubiquitination in fetal microglial inflammation and consequently neuronal DNA damage caused by exposure to ZnO-NPs during pregnancy
Application : IHC-P
Reactivity : Mouse
Hwang TI et al. Int J Oncol 2023; 62 (5) Tumor suppressive functions of hsa?miR?34a on cell cycle, migration and protective autophagy in bladder cancer.
Duan C et al. Front Cell Infect Microbiol 2023; 13:1190602 Fucose ameliorates the proinflammatory property of Fusobacterium nucleatum in colitis via altering its metabolism.
Jiang B et al. Mol Med Rep 2023; 27 (2) Combination of chloroquine diphosphate and salidroside induces human liver cell apoptosis via regulation of mitochondrial dysfunction and autophagy.
Cui Y et al. BMC Vet Res 2022; 18 (1):307 The combination of high glucose and LPS induces autophagy in bovine kidney epithelial cells via the Notch3/mTOR signaling pathway.
Application : WB
Reactivity : Bovine
Miao CC et al. Autophagy 2022; 18 (4):921-934 LC3A-mediated autophagy regulates lung cancer cell plasticity.
Application : WB
Reactivity : Human
Mostafa DK et al. Inflammopharmacology 2022; 30 (6):2521-2535
Kao CH et al. Autophagy 2022; 18 (12):2830-2850 TFEB- and TFE3-dependent autophagy activation supports cancer proliferation in the absence of centrosomes.
Yaocheng Cui et al. Molecules 2022; 27 (10) Notch3-Mediated mTOR Signaling Pathway Is Involved in High Glucose-Induced Autophagy in Bovine Kidney Epithelial Cells.
Application : WB
Reactivity : Bovine
Yan Guo et al. iScience 2022; 25 (7):104519 Histone H2A ubiquitination resulting from Brap loss of function connects multiple aging hallmarks and accelerates neurodegeneration.
Reactivity : Mouse
Lee YG et al. Sci Rep 2022; 12 (1):4673 L-Carnitine reduces reactive oxygen species/endoplasmic reticulum stress and maintains mitochondrial function during autophagy-mediated cell apoptosis in perfluorooctanesulfonate-treated renal tubular cells.
Application : WB
Reactivity : Rat
Chung WP et al. Sci Rep 2022; 12 (1):241 FTY720 in resistant human epidermal growth factor receptor 2-positive breast cancer.
Application : WB
Reactivity : Human
Lee CW et al. Molecules 2022; 27 (2):373 Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.
Application : WB
Reactivity : Human
Kuo-Hao Ho et al. Neurotherapeutics 2021; 18 (2):1371-1392 Guanabenz Sensitizes Glioblastoma Cells to Sunitinib by Inhibiting GADD34-Mediated Autophagic Signaling.
Application : WB
Reactivity : Human
Tung Chao et al. Autophagy 2021; 17 (11):3444-3460 Autophagy restricts mitochondrial DNA damage-induced release of ENDOG (endonuclease G) to regulate genome stability.
Application : WB, IHC-P
Reactivity : Human, Mouse
St?phanie Torrino et al. Elife 2021; 10 UBTD1 regulates ceramide balance and endolysosomal positioning to coordinate EGFR signaling.
Application : WB
Reactivity : Human
Qing Yu et al. Front Pharmacol 2021; 12:658677 Dexmedetomidine Protects Against Septic Liver Injury by Enhancing Autophagy Through Activation of the AMPK/SIRT1 Signaling Pathway.
Application : WB
Reactivity : Mouse
Valentina Naef et al. Int J Mol Sci 2021; 22 (16) Efficient Neuroprotective Rescue of Sacsin-Related Disease Phenotypes in Zebrafish.
Application : WB
Reactivity : Zebrafish
Agote-Ar?n A et al. Front Cell Dev Biol 2021; 9:755847 Fragile X-Related Protein 1 Regulates Nucleoporin Localization in a Cell Cycle-Dependent Manner.
Daussy CF et al. Autophagy 2021; 17 (9):2465-2474 HIV-1 Env induces pexophagy and an oxidative stress leading to uninfected CD4+ T cell death.
Siadous FA et al. Autophagy 2021; 17 (3):706-722 Coxiella effector protein CvpF subverts RAB26-dependent autophagy to promote vacuole biogenesis and virulence.
Watanabe M et al. Molecules 2021; 27 (1) GIF-2209, an Oxindole Derivative, Accelerates Melanogenesis and Melanosome Secretion via the Modification of Lysosomes in B16F10 Mouse Melanoma Cells.
Application : WB
Reactivity : Mouse
Lin CY et al. Antioxidant 2021; 10 (12):1898 Glucose Activates Lysine-Specific Demethylase 1 through the KEAP1/p62 Pathway.
Application : WB, PLA
Reactivity : Human
Chen WT et al. Cancers (Basel) 2021; 13 (16) Serum DJ-1 Is a Biomarker of Colorectal Cancer and DJ-1 Activates Mitophagy to Promote Colorectal Cancer Progression.
Application : WB
Reactivity : Human
Tsai CL et al. J Pers Med 2021; 11 (9) GPR30 Activation by 17β-Estradiol Promotes p62 Phosphorylation and Increases Estrogen Receptor α Protein Expression by Inducing Its Release from a Complex Formed with KEAP1.
Application : WB
Reactivity : Human
Tsai CH et al. Food Chem Toxicol 2021; 154:112318 Docosahexaenoic acid promotes the formation of autophagosomes in MCF-7 breast cancer cells through oxidative stress-induced growth inhibitor 1 mediated activation of AMPK/mTOR pathway.
Application : WB
Reactivity : Human
Chen CY et al. Sci Rep 2021; 11 (1):2377 Cardiac-specific microRNA-125b deficiency induces perinatal death and cardiac hypertrophy.
Application : WB
Reactivity : Mouse
Liu CH et al. Cancers (Basel) 2021; 13 (1) Ursolic Acid and Its Nanoparticles Are Potentiators of Oncolytic Measles Virotherapy against Breast Cancer Cells.
Application : WB
Reactivity : Human
Martina Moras et al. Int J Mol Sci 2020; 21 (23) Downregulation of Mitochondrial TSPO Inhibits Mitophagy and Reduces Enucleation during Human Terminal Erythropoiesis.
Application : WB
Reactivity : Human
Cheung CHY et al. J Biomed Sci 2020; 27 (1):75 ZNF322A-mediated protein phosphorylation induces autophagosome formation through modulation of IRS1-AKT glucose uptake and HSP-elicited UPR in lung cancer.
Martello A et al. EMBO Rep 2020; 21 (7):e48192 Trichoplein binds PCM1 and controls endothelial cell function by regulating autophagy.
Huang HT et al. Antioxidants (Basel) 2020; 10 (1) Intra-Articular Injection of (-)-Epigallocatechin 3-Gallate to Attenuate Articular Cartilage Degeneration by Enhancing Autophagy in a Post-Traumatic Osteoarthritis Rat Model.
Application : IHC-P
Reactivity : Rat
Wong BS et al. Cancer Manag Res 2020; 12:1913-1927 Anticancer Effects of Antihypertensive L-Type Calcium Channel Blockers on Chemoresistant Lung Cancer Cells via Autophagy and Apoptosis.
Mohapatra S et al. Cell Death Differ 2020; (Epub) Estrogen and estrogen receptors chauffeur the sex-biased autophagic action in liver.
Application : WB
Chou YJ et al. Sci Rep 2020; 10 (1):8422 Vaccine adjuvant activity of a TLR4-activating synthetic glycolipid by promoting autophagy.
Application : WB
Reactivity : Mouse
Jensen BK et al. EMBO Mol Med 2020; 12 (5):e10722 Synaptic dysfunction induced by glycine-alanine dipeptides in C9orf72-ALS/FTD is rescued by SV2 replenishment.
Application : ICC/IF
Reactivity : Human
Weng SC et al. Insect Mol Biol 2020; 29 (4):404-416 The unfolded protein response modulates the autophagy-mediated egg production in the mosquito Aedes aegypti.
Application : WB
Reactivity : Mosquito
Saito Y et al. Free Radic Biol Med 2020; 152:235-247 A triterpenoid Nrf2 activator, RS9, promotes LC3-associated phagocytosis of photoreceptor outer segments in a p62-independent manner.
Application : WB
Reactivity : Human
Ho KH et al. Neurotherapeutics 2020; (Epub) A Key Role of DNA Damage-Inducible Transcript 4 (DDIT4) Connects Autophagy and GLUT3-Mediated Stemness To Desensitize Temozolomide Efficacy in Glioblastomas.
Application : WB
Reactivity : Human
Lin MW et al. Neurotherapeutics 2019; (Epub) Galantamine Inhibits Aβ1-42-Induced Neurotoxicity by Enhancing α7nAChR Expression as a Cargo Carrier for LC3 Binding and Aβ1-42 Engulfment During Autophagic Degradation.
Application : WB
Reactivity : Human
Lin MW et al. Antioxidants (Basel) 2019; 9 (1) Celastrol Inhibits Dopaminergic Neuronal Death of Parkinson's Disease through Activating Mitophagy.
Application : WB
Reactivity : Human
Chen JC et al. Biomolecules 2019; 9 (9) In Vitro and In Silico Mechanistic Insights into miR-21-5p-Mediated Topoisomerase Drug Resistance in Human Colorectal Cancer Cells.
Application : WB
Reactivity : Human
Lin TY et al. Autophagy 2019;:1-18 BIRC5/Survivin is a novel ATG12-ATG5 conjugate interactor and an autophagy-induced DNA damage suppressor in human cancer and mouse embryonic fibroblast cells.
Application : WB
Reactivity : Human
Tan X et al. In Vitro Cell Dev Biol Anim 2019; (Epub) SQSTM1/p62 is involved in docosahexaenoic acid-induced cellular autophagy in glioblastoma cell lines.
Application : WB
Reactivity : Human
Wang J et al. Neoplasia 2019; 21 (9):908-920 p85α Inactivates MMP-2 and Suppresses Bladder Cancer Invasion by Inhibiting MMP-14 Transcription and TIMP-2 Degradation.
Application : WB
Reactivity : Human
Hsieh CC et al. Br J Pharmacol 2019; (Epub) Mitochondrial protection by simvastatin against Angiotensin II-mediated heart failure.
Application : WB
Reactivity : Human
Wang YY et al. Oral Dis 2019; 25 (3):758-771 MAL-PDT inhibits oral precancerous cells and lesions via autophagic cell death.
Application : WB
Reactivity : Human
Peng X et al. Cell Death Dis 2019; 10 (4):253 ATG5-mediated autophagy suppresses NF-κB signaling to limit epithelial inflammatory response to kidney injury.
Application : WB
Reactivity : Mouse
Liao CC et al. Autophagy 2018; 14 (12):2065-2082 Autophagic degradation of SQSTM1 inhibits ovarian cancer motility by decreasing DICER1 and AGO2 to induce MIRLET7A-3P.
Lin SY et al. Autophagy 2018; 14 (5):778-795 Necroptosis promotes autophagy-dependent upregulation of DAMP and results in immunosurveillance.
Yi H et al. Molecules 2018; 23 (6) Aleuritolic Acid Impaired Autophagic Flux and Induced Apoptosis in Hepatocellular Carcinoma HepG2 Cells.
Lin KL et al. Free Radic Res 2018; 52 (11-12):1371-1386 Resveratrol provides neuroprotective effects through modulation of mitochondrial dynamics and ERK1/2 regulated autophagy.
Application : WB
Reactivity : Human
Liu YH et al. Phytomedicine 2018; 46:184-192 Aqueous extracts of Paeonia suffruticosa modulates mitochondrial proteostasis by reactive oxygen species-induced endoplasmic reticulum stress in pancreatic cancer cells.
Application : WB
Reactivity : Human
Zheng ZY et al. Sci Rep 2018; 8 (1):16932
Application : WB
Reactivity : Human
Ko JL et al. Environ Toxicol 2018; (Epub) Effects and mechanisms of betulinic acid on improving EGFR TKI-resistance of lung cancer cells.
Application : WB
Reactivity : Human
Chiu YH et al. Int J Oncol 2018; (Epub) Human non-small cell lung cancer cells can be sensitized to camptothecin by modulating autophagy.
Yinhui Li et al. ACS OMEGA 2018; 3 (6):pp 6812–6819 Effective Theranostic Cyanine for Imaging of Amyloid Species in Vivo and Cognitive Improvements in Mouse Model.
Peng M et al. Oncogene 2018; (Epub) PHLPP2 stabilization by p27 mediates its inhibition of bladder cancer invasion by promoting autophagic degradation of MMP2 protein.
Application : WB
Reactivity : Mouse
Liu GT et al. FASEB J 2018;:fj201701253R Improving nuclear envelope dynamics by EBV BFRF1 facilitates intranuclear component clearance through autophagy.
Application : ICC/IF
Chen CH et al. J Appl Physiol 2018; (Epub) Parathyroid hormone (1-34) ameliorated knee osteoarthritis in rats via autophagy.
Huang WT et al. Front Pharmacol 2017; 8:902 HDAC2 and HDAC5 Up-Regulations Modulate Survivin and miR-125a-5p Expressions and Promote Hormone Therapy Resistance in Estrogen Receptor Positive Breast Cancer Cells.
Chen CH et al. Clin Cancer Res 2017; (Epub) Dual Inhibition of PIK3C3 and FGFR as a New Therapeutic Approach to Treat Bladder Cancer.
Application : WB
Hsin MC et al. Cell Death Dis 2017; 8 (10):e3089 Hispolon suppresses metastasis via autophagic degradation of cathepsin S in cervical cancer cells.
Application : WB
Reactivity : Human
Lin LC et al. J Funct Foods 2017; 33:332-344 The licorice dietary component isoliquiritigenin chemosensitizes human uterine sarcoma cells to doxorubicin and inhibits cell growth by inducing apoptosis and autophagy via inhibition of m-TOR signaling
Liu YH et al. Sci Rep 2017; 7:41437 Aqueous extract of Polygonum bistorta modulates proteostasis by ROS-induced ER stress in human hepatoma cells.
Application : WB
Reactivity : Human
Hai J et al. Sci Rep 2017; 7:39692 TRIM14 is a Putative Tumor Suppressor and Regulator of Innate Immune Response in Non-Small Cell Lung Cancer.
Application : WB
Reactivity : Human
Lappas M et al. Reprod Sci 2017; 24 (5):762-772 The Adaptor Protein p62 Mediates Nuclear Factor κB Activation in Response to Inflammation and Facilitates the Formation of Prolabor Mediators in Human Myometrium.
Application : WB
Reactivity : Human
Hsin IL et al. Phytomedicine 2016; 23 (13):1566–1573
Application : WB
Reactivity : Human
Tsai JH et al. Int J Mol Sci 2016; 17 (8) 1-(2-Hydroxy-5-methylphenyl)-3-phenyl-1,3-propanedione Induces G1 Cell Cycle Arrest and Autophagy in HeLa Cervical Cancer Cells.
Application : WB
Reactivity : Human
Tsai WT et al. J Biol Chem 2016; 291 (37):19299-311 Mycotoxin Patulin Suppresses Innate Immune Responses by Mitochondrial Dysfunction and p62/Sequestosome-1-dependent Mitophagy.
Application : WB
Reactivity : Mouse
Li H et al. Autophagy 2016; (Epub) Atg5-mediated autophagy deficiency in proximal tubules promotes cell cycle G2/M arrest and renal fibrosis.
Application : WB, IHC-P
Reactivity : Mouse
Hsu CY et al. Biogerontology 2016; (Epub) Cellular degradation activity is maintained during aging in long-living queen bees.
Application : WB
Reactivity : Honeybee
Hsieh YY et al. Cell Death Discovery 2016; 2:16027 Repositioning of a cyclin-dependent kinase inhibitor GW8510 as a ribonucleotide reductase M2 inhibitor to treat human colorectal cancer.
Application : WB
Reactivity : Human
Chiu LY et al. PLoS One 2015; 10 (5):e0125774 Immunomodulatory Protein from Ganoderma microsporum Induces Pro-Death Autophagy through Akt-mTOR-p70S6K Pathway Inhibition in Multidrug Resistant Lung Cancer Cells.
Application : WB
Reactivity : Human
Liu TP et al. Anticancer Drugs 2015; 26 (2):139-47 S-Adenosyl-L-methionine-competitive inhibitors of the histone methyltransferase EZH2 induce autophagy and enhance drug sensitivity in cancer cells.
Application : WB
Reactivity : Human
Huang CC et al. J Cell Sci 2014; 127 (Pt 14):3024-38 Metabolism and mis-metabolism of the neuropathological signature protein TDP-43.
Application : WB
Reactivity : Human
Li KC et al. Mol Cancer 2014; 13:172 Inhibition of G9a induces DUSP4-dependent autophagic cell death in head and neck squamous cell carcinoma.
Application : WB
Reactivity : Human
Li JR et al. Anticancer Research 2014; 34 (6):2973-2983
Hsu CY et al. Exp Gerontol 2014; 50:128-36 Changes in cellular degradation activity in young and old worker honeybees (Apis mellifera).
Application : WB
Reactivity : Honeybee
Tung YT et al. Mol Neurobiol 2014; 49 (1):43400 Presenilin-1 Regulates the Expression of p62 to Govern p62-dependent Tau Degradation.
Application : WB
Reactivity : Human
Tseng AH et al. Free Radic Biol Med 2013; 63:222-34 SIRT3 deacetylates FOXO3 to protect mitochondria against oxidative damage.
Application : ICC/IF
Reactivity : Bovine
Li JR et al. Toxicol Lett 2013; 220 (3):267-76
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
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