GeneTex
United States (US)

SRPR alpha antibody

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Cat No. GTX16145

Host Sheep
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Dog
APPLICATION

Application Note

IP: Use at an assay dependent dilution. Can be used to immunoprecipitate both subunits of SRPR under non-denaturing conditions. WB: Use at a concentration of 1 - 5 μg/ml. Detects a band of approximately 72 kDa. Optimal dilutions/concentrations should be determined by the end user.

Positive Control

Canine microsomal protein.

Specificity/Sensitivity

This antibody can immunoprecipitate both SRPR alpha and beta under non denaturing conditions.
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.08% Sodium Azide; Constituents: PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dog

Immunogen

Recombinant fragment, corresponding to amino acids 39-295 of Dog SRPR alpha, which includes part of the amino terminal SRPR beta binding region and the hinge region between it and the carboxyl-terminal GTPase domain.

Purification

IgG fraction
This antibody is provided as a 0.2 μm sterile filtered solution.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Srp Receptor Subunit Alpha,Dp-Alpha,Sr-Alpha,Srpr,Srpra

Cellular Localization

Endoplasmic reticulum membrane. Thought to be anchored in the membrane through an interaction with SRPR beta, which contains a bona fide transmembrane domain.

Background

The receptor for Signal Recognition Particle (SRP) is the site on the endoplasmic reticulum that ribosomes translating secreted and integral membrane proteins are initially targeted to. Once the ribosome-SRP complex arrives at the SRP receptor the protein being translated is transferred to the translocation complex (Sec61) in the ER membrane. SRPR is composed of two subunits SRPR alpha and SRPR beta. The SRPR alpha subunit is a translocation GTPase peripherally bound to the endoplasmic reticulum by its interaction with SRPR beta. SRPR alpha also binds to the GTPase of SRP (SRP54) and these two proteins appear to function as each others GTPase activating proteins (GAPs). Hydrolysis of GTP by SRPR alpha and SRP54 is thought to be involved in transfer of the nascent protein to the Sec61 complex in the ER. SRPR alpha has an apparent molecular weight of 72 kDa. SRPR beta is a Type I transmembrane protein that spans the membrane once and contains Ras type GTPase domain. The function of the GTPase in SRPR beta is unknown. The membrane spanning domain is at the amino-terminus of SRPR beta. SRPR alpha binds to the GTPase domain of SRPR beta. Heterodimerization of SRPR alpha and SRPR beta masks the carboxyl-terminal epitope of SRPR beta.
DATA IMAGES
img25880_18121411_965.jpg

GTX16145 WB Image

SRPR alpha antibody

Package List Price ($)
$ 329