Immunofluorescent analysis of 4% PFA fixed human embryonal carcinoma cell line Tera using anti-SSEA-5 Ab (1:1000 dilution). SSEA-5 positive cells (red) represented pluripotent population; negative cells represented differentiated population. DAPI (Blue) indicated nuclear staining.

Pluripotent stem cell population indicated by SSEA5-staining. Human embryonic stem cells H9 were analyzed with SSEA-5 mAB (pseudo-colored in red) and control IgG (pseudo-colored in green) in flow cytometry. Each Ab was used at a 1:50 dilution in PBS.

Immunofluorescent analysis of 4% FA fixed human embryonic stem cells H9 using anti-SSEA-5 Ab (1:50 dilution).
(A) Brightfield image
(B) Dapi
(C) anti-SSEA-5 Ab
(D) Merged image of anti-SSEA-5 Ab and DAPI