APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:1000-1:2000 |
1:50-1:100 |
1:50 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.7 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length human recombinant protein of human STAT1 (NP_009330) produced in HEK293T cell.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
signal transducer and activator of transcription 1 , CANDF7 , IMD31A , IMD31B , IMD31C , ISGF-3 , STAT91
Cellular Localization
Cytoplasm,Nucleus
Background
Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.
Database
Research Area
DATA IMAGES
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GTX83552 WB Image
WB analysis of HEK293T cells transfected with STAT1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83552 STAT1 antibody [4H9]. Loading : 5 ug per lane
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GTX83552 ICC/IF Image
ICC/IF analysis of COS7 cells transiently transfected with STAT1 plasmid using GTX83552 STAT1 antibody [4H9].
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GTX83552 IHC-P Image
IHC-P analysis of human endometrium tissue using GTX83552 STAT1 antibody [4H9]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
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GTX83552 WB Image
WB analysis of various cell lines using GTX83552 STAT1 antibody [4H9]. Loading : 35 ug per lane
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GTX83552 ICC/IF Image
ICC/IF analysis of HT29 cells using GTX83552 STAT1 antibody [4H9].
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GTX83552 IHC-P Image
IHC-P analysis of human kidney tissue using GTX83552 STAT1 antibody [4H9]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
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GTX83552 WB Image
WB analysis of human tissues (Lane 1-Uterus ; Lane 2-Breast ; Lane 3-Brain ; Lane 4-Liver ; Lane 5-Ovary ; Lane 6-Thyroid gland ; Lane 7-colon) using GTX83552 STAT1 antibody [4H9]. Loading : 10 ug per lane Dilution : 1:200
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GTX83552 IHC-P Image
IHC-P analysis of human thyroid carcinoma tissue using GTX83552 STAT1 antibody [4H9]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
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GTX83552 IHC-P Image
IHC-P analysis of human breast tissue using GTX83552 STAT1 antibody [4H9]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
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REFERENCE
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REVIEW
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