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STAT1 antibody [4H9]

Overexpression
Anti-STAT1 antibody [4H9] used in Western Blot (WB). GTX83552
Anti-STAT1 antibody [4H9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83552
Anti-STAT1 antibody [4H9] used in IHC (Paraffin sections) (IHC-P). GTX83552
Anti-STAT1 antibody [4H9] used in Western Blot (WB). GTX83552
Anti-STAT1 antibody [4H9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83552
Anti-STAT1 antibody [4H9] used in IHC (Paraffin sections) (IHC-P). GTX83552
Anti-STAT1 antibody [4H9] used in Western Blot (WB). GTX83552
Anti-STAT1 antibody [4H9] used in IHC (Paraffin sections) (IHC-P). GTX83552
Anti-STAT1 antibody [4H9] used in IHC (Paraffin sections) (IHC-P). GTX83552

Cat No. GTX83552

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4H9

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Dog, Monkey
Package
100 μl ($409)
See all STAT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:2000
ICC/IF 1:50-1:100
IHC-P 1:50
Not tested in other applications.

Calculated MW

87 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human STAT1 (NP_009330) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

signal transducer and activator of transcription 1 , CANDF7 , IMD31A , IMD31B , IMD31C , ISGF-3 , STAT91

Cellular Localization

Cytoplasm,Nucleus

Background

Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.

Database

Research Area

DATA IMAGES

Anti-STAT1 antibody [4H9] used in Western Blot (WB). GTX83552

GTX83552 WB Image

Overexpression

WB analysis of HEK293T cells transfected with STAT1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83552 STAT1 antibody [4H9].
Loading : 5 ug per lane

Anti-STAT1 antibody [4H9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83552

GTX83552 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with STAT1 plasmid using GTX83552 STAT1 antibody [4H9].

Anti-STAT1 antibody [4H9] used in IHC (Paraffin sections) (IHC-P). GTX83552

GTX83552 IHC-P Image

IHC-P analysis of human endometrium tissue using GTX83552 STAT1 antibody [4H9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-STAT1 antibody [4H9] used in Western Blot (WB). GTX83552

GTX83552 WB Image

WB analysis of various cell lines using GTX83552 STAT1 antibody [4H9].
Loading : 35 ug per lane

Anti-STAT1 antibody [4H9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83552

GTX83552 ICC/IF Image

ICC/IF analysis of HT29 cells using GTX83552 STAT1 antibody [4H9].

Anti-STAT1 antibody [4H9] used in IHC (Paraffin sections) (IHC-P). GTX83552

GTX83552 IHC-P Image

IHC-P analysis of human kidney tissue using GTX83552 STAT1 antibody [4H9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-STAT1 antibody [4H9] used in Western Blot (WB). GTX83552

GTX83552 WB Image

WB analysis of human tissues (Lane 1-Uterus ; Lane 2-Breast ; Lane 3-Brain ; Lane 4-Liver ; Lane 5-Ovary ; Lane 6-Thyroid gland ; Lane 7-colon) using GTX83552 STAT1 antibody [4H9].
Loading : 10 ug per lane
Dilution : 1:200

Anti-STAT1 antibody [4H9] used in IHC (Paraffin sections) (IHC-P). GTX83552

GTX83552 IHC-P Image

IHC-P analysis of human thyroid carcinoma tissue using GTX83552 STAT1 antibody [4H9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-STAT1 antibody [4H9] used in IHC (Paraffin sections) (IHC-P). GTX83552

GTX83552 IHC-P Image

IHC-P analysis of human breast tissue using GTX83552 STAT1 antibody [4H9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

REFERENCE

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REVIEW

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Package List Price ($)
$ 409