Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using STAT3 (Phospho Y705)(GTX118000) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Untreated (–) and treated (+) A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT3 (phospho Tyr705) antibody (GTX118000) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT3 (phospho Tyr705) antibody (GTX118000) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal Int J Mol Sci in 2020.PMID: 32059373

The data was published in the journal Int J Mol Sci in 2020.PMID: 32059373