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STAT5A antibody [C2C3], C-term

Anti-STAT5A antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113351
Anti-STAT5A antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX113351
Anti-STAT5A antibody [C2C3], C-term used in Western Blot (WB). GTX113351
Anti-STAT5A antibody [C2C3], C-term used in Western Blot (WB). GTX113351
Anti-STAT5A antibody [C2C3], C-term used in Western Blot (WB). GTX113351
Anti-STAT5A antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX113351

Cat. No. GTX113351

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

91 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , OECM-1 , SCC-9

Predict Reactivity

Rat, Sheep, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human STAT5A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176176

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

signal transducer and activator of transcription 5A , MGF , STAT5

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-STAT5A antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113351

GTX113351 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using STAT5A(GTX113351) antibody at 1:500 dilution.

Anti-STAT5A antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX113351

GTX113351 IHC-P Image

STAT5A antibody [C2C3], C-term detects STAT5A protein at cytosol and nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
STAT5A antibody [C2C3], C-term (GTX113351) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-STAT5A antibody [C2C3], C-term used in Western Blot (WB). GTX113351

GTX113351 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
7.5% SDS PAGE
GTX113351 diluted at 1:1000

Anti-STAT5A antibody [C2C3], C-term used in Western Blot (WB). GTX113351

GTX113351 WB Image

Whole cell extract (12.7 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT5A antibody [C2C3], C-term (GTX113351) diluted at 1:5000.

Anti-STAT5A antibody [C2C3], C-term used in Western Blot (WB). GTX113351

GTX113351 WB Image

Whole cell extract (22.5 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT5A antibody [C2C3], C-term (GTX113351) diluted at 1:2000.

Anti-STAT5A antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX113351

GTX113351 IHC-P Image

STAT5A antibody [C2C3], C-term detects STAT5A protein at cytosol and nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
STAT5A antibody [C2C3], C-term (GTX113351) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

STAT5A antibody [C2C3], C-term Cat. No. GTX113351

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 05-Jul-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : HSC-3 cells
Amount used : 15μg
Blocking : 5% milk in 0.1% TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169