STAT5A antibody [C2C3], C-term

Name: STAT5A antibody [C2C3], C-term
Brand: GeneTex
SKU: GTX113351
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-STAT5A antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113351

Anti-STAT5A antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX113351

Anti-STAT5A antibody [C2C3], C-term used in Western Blot (WB). GTX113351

Cat. No. GTX113351

Reference 2

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all STAT5A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

91 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , OECM-1 , SCC-9

Predict Reactivity

Rat, Sheep, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human STAT5A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176176

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

signal transducer and activator of transcription 5A , MGF , STAT5

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq]

Database

Gene ID: 6776 STAT5A
UniProt: P42229 STAT5A

Research Area

DATA IMAGES

GTX113351 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using STAT5A(GTX113351) antibody at 1:500 dilution.

GTX113351 IHC-P Image

STAT5A antibody [C2C3], C-term detects STAT5A protein at cytosol and nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
STAT5A antibody [C2C3], C-term (GTX113351) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX113351 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
7.5% SDS PAGE
GTX113351 diluted at 1:1000

GTX113351 WB Image

Whole cell extract (12.7 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT5A antibody [C2C3], C-term (GTX113351) diluted at 1:5000.

GTX113351 WB Image

Whole cell extract (22.5 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT5A antibody [C2C3], C-term (GTX113351) diluted at 1:2000.

GTX113351 IHC-P Image

STAT5A antibody [C2C3], C-term detects STAT5A protein at cytosol and nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
STAT5A antibody [C2C3], C-term (GTX113351) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Chi M et al. Sci Rep 2016; 6 : 33132 Phosphorylation of calcium/calmodulin-stimulated protein kinase II at T286 enhances invasion and migration of human breast cancer cells.

Application : WB

Reactivity : Human

Lee MC et al. J Leukoc Biol 2014; 95 (1) : 105-15 Gfi-1 is the transcriptional repressor of SOCS1 in acute myeloid leukemia cells.

Application : WB

Reactivity : Human

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