APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:10000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , OECM-1 , SCC-9
Predict Reactivity
Rat, Sheep, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris-Glycine (pH7), 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human STAT5A. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11176176
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
signal transducer and activator of transcription 5A , MGF , STAT5
Cellular Localization
Cytoplasm , Nucleus
Background
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX113351 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using STAT5A(GTX113351) antibody at 1:500 dilution.
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GTX113351 IHC-P Image
STAT5A antibody [C2C3], C-term detects STAT5A protein at cytosol and nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. STAT5A antibody [C2C3], C-term (GTX113351) dilution: 1:250.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX113351 WB Image
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7.5% SDS PAGE GTX113351 diluted at 1:1000
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GTX113351 WB Image
Whole cell extract (12.7 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT5A antibody [C2C3], C-term (GTX113351) diluted at 1:5000.
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GTX113351 WB Image
Whole cell extract (22.5 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT5A antibody [C2C3], C-term (GTX113351) diluted at 1:2000.
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GTX113351 IHC-P Image
STAT5A antibody [C2C3], C-term detects STAT5A protein at cytosol and nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. STAT5A antibody [C2C3], C-term (GTX113351) dilution: 1:250.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
REVIEW
STAT5A antibody [C2C3], C-term
Cat. No. GTX113351
Rating
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( Average 4 based on 1 users reviews)
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Application
Western Blot(WB)
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( Average 4 based on 1 users reviews)
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Date :
Anonymous submitted on 05-Jul-2016
Application Tested :
WB
Sample Species :
Hu
Sample :
HSC-3 cells
Amount used :
15μg
Blocking :
5% milk in 0.1% TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr