WB (peptide competition) analysis of 3T3-L1 cells treated with 20 ng/mL LIF for 10 minutes (Lane 2-5) using GTX13593 STAT5A (phospho Tyr694) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 3), a generic phosphotyrosine-containing peptide (Lane 4), or the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

ChIP analysis of 2 million HeLa cells treated with 100ng/ml of IFN gamma for 45 minutes using GTX13593 STAT5A (phospho Tyr694) antibody. Normal Rabbit IgG was used as a negative IP control. The precipitated DNA was detected by PCR with primer set targeting to the promoter of COX1, AP1 gene as positive control target and the inactive SAT2 used as negative control target.

IHC-P analysis of mouse spleen tissue using GTX13593 STAT5A (phospho Tyr694) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

ICC/IF analysis of HeLa cells treated with 5uM of TNF-alpha for 30 minutes using GTX13593 STAT5A (phospho Tyr694) antibody. Panel e is untreated cell with no signal. Panel f is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Trito X-100 for 10 minutesDilution: 1:250