GeneTex
United States (US)

STK3 (Active) recombinant protein

Cat No. GTX65261

Application ELISA, Functional Assay, Apuri, Blocking
Reactivity Human
Species Human
APPLICATION

Application Note

277 nmol phosphate incorporated into MBP per minute per mg protein at 30ºC for 15 minutes using a final concentration of 50 uM ATP (0.83 uCi/assay).

Calculated MW

87.0 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol

Storage

Store at -80ºC. Product is stable for at least 6-12 months.

Concentration

0.1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Expression System

Baculovirus (Sf9 insect cells)

Purification


Purity was assessed by SDS-PAGE (≥90%) and by HPLC.

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

serine/threonine kinase3, serine/threonine kinase 3, STK, STK3

Background

Creasy identified a cDNA encoding STK3, which they called MST2. Sequence analysis indicated that the deduced 491-amino acid STK3 protein contains an N-terminal catalytic domain characteristic of STKs (1). Taylor also isolated cDNAs encoding STK3 (p63) and STK4 (p61) respectively. STK3 and STK4 share 94% amino acid identity in the catalytic domain and 78% identity overall (2). The yeast 'sterile 20' (Ste20) kinase acts upstream of the mitogen-activated protein kinase (MAPK) cascade that is activated under a variety of stress conditions. O'Neill used proteomic analysis of RAF1 signaling complexes to show that RAF1 counteracts apoptosis by suppressing the activation of mammalian sterile 20-like kinase (MST2) (3). MST2 was identified as a kinase that is activated by the proapoptotic agents straurosporine and FAS ligand (2; 4). STK3 activation presumably allows cells to resist unfavorable environmental conditions.

Research Area

Package List Price ($)
$ 349