SUMO1 antibody [SM1/495]

Cat. No. GTX35092

Host	Mouse
Clonality	Monoclonal
Clone Name	SM1/495
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human, Rat
Package	100 μg ($529)

See all SUMO1 products

Datasheet
Document

View product citations for antibody GTX35092 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1-2μg/ml
ICC/IF	1-2μg/ml
IHC-P	1-2μg/ml for 30 minutes at RT
FACS	1-2μg/10⁶ cells
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

12 kDa. ( Note )

Product Note

This MAb is specific to SUMO-1 and shows no cross-reaction with either SUMO-2 or SUMO-3.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human SUMO1 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DAP1 , GMP1 , OFC10 , PIC1 , SENP2 , SMT3 , SMT3C , SMT3H3 , SUMO1 , UBL1 , small ubiquitinlike modifier 1 , Sumo 1 , small ubiquitin like modifier 1

Cellular Localization

Nucleus membrane,Nucleus speckle,Cytoplasm,Nucleus,PML body

Background

This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Jul 2008]

Database

Gene ID: 7341 SUMO1
UniProt: P63165 SUMO1

Research Area

DATA IMAGES

GTX35092 ICC/IF Image

ICC/IF analysis of paraformaldehyde-fixed HepG2 cells using GTX35092 SUMO1 antibody [SM1/495].
Green : Primary antibody
Red : Phalloidin

GTX35092 FACS Image

FACS analysis of paraformaldehyde-fixed HepG2 cells using GTX35092 SUMO1 antibody [SM1/495].
Blue : Primary antibody
Red : Isotype control

GTX35092 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX35092 SUMO1 antibody [SM1/495].

GTX35092 WB Image

WB analysis of HeLa cell lysate using GTX35092 SUMO1 antibody [SM1/495].

REFERENCE

There are currently no references for SUMO1 antibody [SM1/495] (GTX35092). Be the first to share your publications with this product.

Submit a Reference

REVIEW

There are currently no reviews for SUMO1 antibody [SM1/495] (GTX35092). Be the first to share your experience with this product.

Submit a Review

SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 529

Qty