United States (US)

SUMO1 antibody [SM1/495]

Anti-SUMO1 antibody [SM1/495] used in Western Blot (WB). GTX35092
Anti-SUMO1 antibody [SM1/495] used in IHC (Paraffin sections) (IHC-P). GTX35092

Cat No. GTX35092

Host Mouse
Clonality Monoclonal
Clone Name SM1/495
Isotype IgG1
Application WB, ICC/IF, IHC-P
Reactivity Human, Rat

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 0.5-1ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

12 kDa. ( Note )


This MAb is specific to SUMO-1 and shows no cross-reaction with either SUMO-2 or SUMO-3. The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, SUMO-2 and SUMO-3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1 protein localizes to the nuclear membrane.




Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.


Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species



Recombinant human SUMO1 protein


Ab purified from Bioreactor Concentrate by Protein A/G




For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.


Small Ubiquitin-Like Modifier 1,Dap1,Gmp1,Ofc10,Pic1,Senp2,Smt3,Smt3C,Smt3H3,Ubl1,Sumo1


This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Jul 2008]


Research Area

Anti-SUMO1 antibody [SM1/495] used in Western Blot (WB). GTX35092

GTX35092 WB Image

Western Blot of HeLa Cell Lysate using SUMO1 Monoclonal Antibody (SM1/495)

Anti-SUMO1 antibody [SM1/495] used in IHC (Paraffin sections) (IHC-P). GTX35092

GTX35092 IHC-P Image

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with SUMO-1 Monoclonal Antibody (SM1/495)

Package List Price ($)
$ 329