Summary
This immunoassay kit allows for the in vitro quantitative determination of general Serotonin concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serotonin, During the reaction, Serotonin in the sample or standard competes with a fixed amount of biotin-labeled Serotonin for sites on a pre-coated Monoclonal antibody specific to Serotonin. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Serotonin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Content
| |
Kit components |
Package |
Storage Temp |
| 1. |
Assay plate |
96 well |
-20ºC |
| 2. |
Standard |
2 vials |
-20ºC |
| 3. |
Sample Diluent |
20ml |
-20ºC |
| 4. |
Assay Diluent A |
10ml |
-20ºC |
| 5. |
Assay Diluent B |
10ml |
-20ºC |
| 6. |
Detection Reagent A |
60μl |
-20ºC |
| 7. |
Detection Reagent B |
120μl |
-20ºC |
| 8. |
Wash Buffer (25 x concentrate) |
30ml |
-20ºC |
| 9. |
Substrate |
10ml |
4-8ºC |
| 10. |
Stop Solution |
10ml |
-20ºC |
| 11. |
Plate sealer for 96 wells |
5 |
-20ºC |
|
Form
Liquid
Storage
The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20ºC upon being received. After receiving the kit , Substrate should be always stored at 4ºC.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20ºC(Except the substrate). The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above. Note: Because of the inherent stability of lyophilized material, GeneTex may ship these materials at ambient temperature.
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
