GeneTex
United States (US)

Somatostatin receptor 1 antibody

GTX130041_41941_20160526_WB_B_18121410_475.jpg
GTX130041_41941_20160630_WB_M_brain_18121410_287.jpg
GTX130041_41941_20171201_IHC-P_M_18121410_723.jpg
GTX130041_41941_20160630_WB_18121410_820.jpg

Cat No. GTX130041

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000

Note :

WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.

Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

HeLa , human SSTR1-transfected 293T cells un-boiled , mouse brain

Predict Reactivity

Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.11 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Somatostatin receptor 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Somatostatin Receptor 1,Srif-2,Ss-1-R,Ss1-R,Ss1R,Sstr1

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Somatostatin acts at many sites to inhibit the release of many hormones and other secretory proteins. The biological effects of somatostatin are probably mediated by a family of G protein-coupled receptors that are expressed in a tissue-specific manner. The encoded protein is a member of the superfamily of somatostatin receptors having seven transmembrane segments, and is expressed in highest levels in jejunum and stomach. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX130041_41941_20160526_WB_B_18121410_475.jpg

GTX130041 WB Image

Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Somatostatin receptor 1 antibody (GTX130041) diluted at 1:1000.

GTX130041_41941_20160630_WB_M_brain_18121410_287.jpg

GTX130041 WB Image

Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Somatostatin receptor 1 antibody (GTX130041) diluted at 1:1000.

GTX130041_41941_20171201_IHC-P_M_18121410_723.jpg

GTX130041 IHC-P Image

Somatostatin receptor 1 antibody detects Somatostatin receptor 1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Somatostatin receptor 1 stained by Somatostatin receptor 1 antibody (GTX130041) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX130041_41941_20160630_WB_18121410_820.jpg

GTX130041 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Somatostatin receptor 1 antibody (GTX130041) diluted at 1:1000.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169