APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
|
Note :
WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.
|
Not tested in other applications.
Calculated MW
Positive Control
human SSTR1-transfected 293T cells un-boiled , mouse brain , HeLa
Predict Reactivity
Rat, Dog, Chicken, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
2.11 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Somatostatin receptor 1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886162
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
somatostatin receptor 1 , SRIF-2 , SS-1-R , SS1-R , SS1R
Cellular Localization
Cell membrane; Multi-pass membrane protein
Background
Somatostatin acts at many sites to inhibit the release of many hormones and other secretory proteins. The biological effects of somatostatin are probably mediated by a family of G protein-coupled receptors that are expressed in a tissue-specific manner. The encoded protein is a member of the superfamily of somatostatin receptors having seven transmembrane segments, and is expressed in highest levels in jejunum and stomach. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX130041 WB Image
Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Somatostatin receptor 1 antibody (GTX130041) diluted at 1:1000.
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GTX130041 WB Image
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Somatostatin receptor 1 antibody (GTX130041) diluted at 1:1000.
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GTX130041 IHC-P Image
Somatostatin receptor 1 antibody detects Somatostatin receptor 1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.Somatostatin receptor 1 stained by Somatostatin receptor 1 antibody (GTX130041) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX130041 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Somatostatin receptor 1 antibody (GTX130041) diluted at 1:1000.
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REFERENCE
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REVIEW
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