APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
|
Note :
WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.
|
Not tested in other applications.
Calculated MW
Positive Control
human SSTR1-transfected 293T cells un-boiled , mouse brain , HeLa
Predict Reactivity
Rat, Dog, Chicken, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
2.11 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide encompassing a sequence within the Intracellular domain of human Somatostatin receptor 1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886162
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
somatostatin receptor 1 , SRIF-2 , SS-1-R , SS1-R , SS1R
Cellular Localization
Cell membrane; Multi-pass membrane protein
Background
Somatostatin acts at many sites to inhibit the release of many hormones and other secretory proteins. The biological effects of somatostatin are probably mediated by a family of G protein-coupled receptors that are expressed in a tissue-specific manner. The encoded protein is a member of the superfamily of somatostatin receptors having seven transmembrane segments, and is expressed in highest levels in jejunum and stomach. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX130041 WB Image
Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Somatostatin receptor 1 antibody (GTX130041) diluted at 1:1000.
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GTX130041 IHC-P Image
Somatostatin receptor 1 antibody detects Somatostatin receptor 1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.Somatostatin receptor 1 stained by Somatostatin receptor 1 antibody (GTX130041) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX130041 WB Image
Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Somatostatin receptor 1 antibody (GTX130041) diluted at 1:1000.
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GTX130041 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Somatostatin receptor 1 antibody (GTX130041) diluted at 1:1000.
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REFERENCE
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REVIEW
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