GeneTex
United States (US)

Somatostatin receptor 1 antibody

Overexpression
GTX130041_41941_20160526_WB_B_18121410_475.jpg
GTX130041_41941_20160630_WB_M_brain_18121410_287.jpg
GTX130041_41941_20171201_IHC-P_M_18121410_723.jpg
GTX130041_41941_20160630_WB_18121410_820.jpg

Cat No. GTX130041

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000

Note :

WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.

Not tested in other applications.

Calculated MW

43 kDa. ( Note )

Positive Control

HeLa , human SSTR1-transfected 293T cells un-boiled , mouse brain

Predict Reactivity

Rat, Bovine, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.11mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Somatostatin receptor 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

SS-1-R antibody , SS1-R antibody , SS1R antibody , SSTR1 antibody , Somatostatin receptor 1 antibody , Somatostatin receptor 1 antibody

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Somatostatin acts at many sites to inhibit the release of many hormones and other secretory proteins. The biological effects of somatostatin are probably mediated by a family of G protein-coupled receptors that are expressed in a tissue-specific manner. The encoded protein is a member of the superfamily of somatostatin receptors having seven transmembrane segments, and is expressed in highest levels in jejunum and stomach. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX130041_41941_20160526_WB_B_18121410_475.jpg

GTX130041 WB Image

Overexpression

Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Somatostatin receptor 1 antibody (GTX130041) diluted at 1:1000.

GTX130041_41941_20160630_WB_M_brain_18121410_287.jpg

GTX130041 WB Image

Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Somatostatin receptor 1 antibody (GTX130041) diluted at 1:1000.

GTX130041_41941_20171201_IHC-P_M_18121410_723.jpg

GTX130041 IHC-P Image

Somatostatin receptor 1 antibody detects Somatostatin receptor 1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Somatostatin receptor 1 stained by Somatostatin receptor 1 antibody (GTX130041) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX130041_41941_20160630_WB_18121410_820.jpg

GTX130041 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Somatostatin receptor 1 antibody (GTX130041) diluted at 1:1000.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169