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Sox2 antibody

Anti-Sox2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX124477
Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477
Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477
Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477
Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477
Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477
Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477

Cat. No. GTX124477

Reference 15
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm

Reactivity

 Zebrafish, Japanese Medaka, Fish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Sox2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr 1:100-1:1000
IHC-Wm 1:50-1:500
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Predict Reactivity

Human (100%)| Mouse (99%)| Rat (99%)| Zebrafish (92%)| Sheep (100%)| Bovine (100%)| Cat (100%)| Chicken (94%)| Pig (98%)| Japanese medaka (88%)| Xenopus tropicalis (90%)| Rhesus Monkey (100%)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.41 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Sox2.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11178063

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SRY (sex determining region Y)-box 2 , cb236 , soxp , wu:fb83g04 , wu:fc14d07 , zgc:65860 , zgc:77389

Cellular Localization

Nucleus , Cytoplasm

Background

Transcriptional activator. May function as a switch in neuronal development.

Database

Research Area

DATA IMAGES

Anti-Sox2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX124477

GTX124477 IHC-Fr Image

Immunohistochemical analysis of frozen sections of zebrafish retina, using sox2 (GTX124477) antibody at 1:1000 dilution.

Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477

GTX124477 IHC-Wm Image

Sox2 antibody detects Sox2 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Sox2 antibody (GTX124477) dilution: 1:50.
Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477

GTX124477 IHC-Wm Image

Sox2 antibody detects Sox2 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Sox2 antibody (GTX124477) dilution: 1:100.
Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477

GTX124477 IHC-Wm Image

Sox2 antibody detects Sox2 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Sox2 antibody (GTX124477) dilution: 1:100.

Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477

GTX124477 IHC-Wm Image

Sox2 antibody detects Sox2 protein on Medaka by whole mount immunohistochemical analysis.
Sample: 7 days-post-fertilization medaka embryo.
Sox2 antibody (GTX124477) dilution: 1:100.
Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477

GTX124477 IHC-Wm Image

Sox2 antibody detects Sox2 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Sox2 antibody (GTX124477) dilution: 1:100.

Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477

GTX124477 IHC-Wm Image

Sox2 antibody detects Sox2 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Sox2 antibody (GTX124477) dilution: 1:100.

REFERENCE

Application Reference

Diego P?rez-Dones et al. Biomedicines 2021; 9 (9) Quantitative Approaches to Study Retinal Neurogenesis.
Reactivity : Zebrafish
Zilova L et al. Elife 2021; 10 Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development.
Application : IHC-Wm
Reactivity : Japanese Medaka
Hardy K et al. J Physiol 2021; 599 (16) : 3913-3936 Functional development and regeneration of hair cells in the zebrafish lateral line.
Reactivity : Zebrafish
Li J et al. Nucleic Acids Res 2021; 49 (4) : 2027-2043 Prpf31 is essential for the survival and differentiation of retinal progenitor cells by modulating alternative splicing.
Application : IHC-Wm
Reactivity : Zebrafish
Ledesma-Terr?n M et al. Development 2020; 147 (14) FGF2 modulates simultaneously the mode, the rate of division and the growth fraction in cultures of radial glia.
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Submit a Reference

REVIEW

Sox2 antibody Cat. No. GTX124477

Rating
( Average 4.7 based on 3 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
IHC (Whole mount)(IHC-Wm)
( Average 4.5 based on 2 users reviews)
Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-Wm
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28717156
Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477
Date : Anonymous submitted on 21-Jul-2021
Score :
Application Tested : IHC-Wm
Sample Species : Zfsh
Sample : Zebrafish embryo, 20 hpf
Blocking : 5% BSA, 0.5% TritonX100, 0.5% Tween20 in PBS, 25°C, 2Hr
Fixation : 4% PFA in PBS
Permeabilization /Antigen retrieval : 1% Triton in PBS
Primary Antibody : 1:200, 4°C, 16Hr
Figure Note : Sox2 antibody detects Sox2 protein (Alexa 488) in the zebrafish neural tube by whole mount immunohistochemical analysis.
Anti-Sox2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124477
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal iScience in 2019. PMID: 31055217
Submit a Review
Application Reference
Diego P?rez-Dones et al. Biomedicines 2021; 9 (9) Quantitative Approaches to Study Retinal Neurogenesis.
Reactivity : Zebrafish
Zilova L et al. Elife 2021; 10 Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development.
Application : IHC-Wm
Reactivity : Japanese Medaka
Hardy K et al. J Physiol 2021; 599 (16):3913-3936 Functional development and regeneration of hair cells in the zebrafish lateral line.
Reactivity : Zebrafish
Li J et al. Nucleic Acids Res 2021; 49 (4):2027-2043 Prpf31 is essential for the survival and differentiation of retinal progenitor cells by modulating alternative splicing.
Application : IHC-Wm
Reactivity : Zebrafish
Ledesma-Terr?n M et al. Development 2020; 147 (14) FGF2 modulates simultaneously the mode, the rate of division and the growth fraction in cultures of radial glia.
Nagashima M et al. J Neurosci 2020; 40 (6):1232-1247 Midkine-a Is Required for Cell Cycle Progression of M?ller Glia during Neuronal Regeneration in the Vertebrate Retina.
Application : IHC-Wm
Reactivity : Zebrafish
Hideki Ando et al. iScience 2019; 15:95-108 Cereblon Control of Zebrafish Brain Size by Regulation of Neural Stem Cell Proliferation.
Application : ICC/IF
Reactivity : Zebrafish
Bloomquist RF et al. Proc Natl Acad Sci U S A 2019; 116 (36):17858-17866 Developmental plasticity of epithelial stem cells in tooth and taste bud renewal.
Application : IHC-P
Reactivity : Fish
Mario Ledesma-Terr?n et al. bioRxiv 2019; (Epub) Radial Glial from mammalian developing neocortex can divide symmetrically in vitro.
Application : ICC/IF
Xia W et al. FEBS Lett 2019; (Epub) Mutations in TOP2B cause autosomal-dominant hereditary hearing loss via inhibition of the PI3K-Akt signalling pathway.
Application : IHC-Wm
Reactivity : Zebrafish
Bayramli X et al. Sci Rep 2017; 7 (1):5572 Patterned Arrangements of Olfactory Receptor Gene Expression in Zebrafish are Established by Radial Movement of Specified Olfactory Sensory Neurons.
Application : IHC-Wm
Reactivity : Zebrafish
Seleit A et al. bioRxiv 2017; (Epub) Neural stem cells induce the formation of their physical niche during organogenesis.
Reactivity : Japanese Medaka
Gorsuch RA et al. Exp Eye Res 2017; (Epub) Sox2 regulates Muller glia reprogramming and proliferation in the regenerating zebrafish retina via Lin28 and Ascl1a.
Application : WB
Reactivity : Zebrafish
Bloomquist, Ryan F et al. 0 2015; Developmental plasticity of stem cells in teeth and taste bud renewal.
Application : IHC-P
Reactivity : Zebrafish
Ogai K et al. Neurosci Res 2014; Function of Sox2 in ependymal cells of lesioned spinal cords in adult zebrafish.
Application : IHC-Wm
Reactivity : Zebrafish
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169