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Sox2 antibody

Anti-Sox2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX124477
Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477
Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477
Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477
Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477
Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477
Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477

Cat No. GTX124477

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm

Reactivity

Zebrafish, Japanese Medaka, Fish
Package
200 μl ($409),
50 μl ($219)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr 1:100-1:1000
IHC-Wm 1:50-1:500
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1XPBS,20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.41 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Sox2.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11178063

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SRY (sex determining region Y)-box 2 , cb236 , soxp , wu:fb83g04 , wu:fc14d07 , zgc:65860 , zgc:77389

Cellular Localization

Nucleus , Cytoplasm

Background

Transcriptional activator. May function as a switch in neuronal development.

Database

Research Area

DATA IMAGES

Anti-Sox2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX124477

GTX124477 IHC-Fr Image

Immunohistochemical analysis of frozen sections of zebrafish retina, using sox2 (GTX124477) antibody at 1:1000 dilution.

Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477

GTX124477 IHC-Wm Image

Sox2 antibody detects Sox2 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Sox2 antibody (GTX124477) dilution: 1:50.

Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477

GTX124477 IHC-Wm Image

Sox2 antibody detects Sox2 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Sox2 antibody (GTX124477) dilution: 1:100.

Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477

GTX124477 IHC-Wm Image

Sox2 antibody detects Sox2 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Sox2 antibody (GTX124477) dilution: 1:100.

Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477

GTX124477 IHC-Wm Image

Sox2 antibody detects Sox2 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Sox2 antibody (GTX124477) dilution: 1:100.

Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477

GTX124477 IHC-Wm Image

Sox2 antibody detects Sox2 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Sox2 antibody (GTX124477) dilution: 1:100.

Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477

GTX124477 IHC-Wm Image

Sox2 antibody detects Sox2 protein on Medaka by whole mount immunohistochemical analysis.
Sample: 7 days-post-fertilization medaka embryo.
Sox2 antibody (GTX124477) dilution: 1:100.

REFERENCE

REVIEW

Sox2 antibody Cat No. GTX124477

Rating ( Average 4.7 based on 3 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
IHC (Whole mount)(IHC-Wm) ( Average 4.5 based on 2 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-Wm
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017.PMID: 28717156
Date : Anonymous submitted on 21-Jul-2021
Score :
Application Tested : IHC-Wm
Sample Species : Zfsh
Sample : Zebrafish embryo, 20 hpf
Blocking : 5% BSA, 0.5% TritonX100, 0.5% Tween20 in PBS, 25°C, 2Hr
Fixation : 4% PFA in PBS
Permeabilization /Antigen retrieval : 1% Triton in PBS
Primary Antibody : 1:200, 4°C, 16Hr
Figure Note : Sox2 antibody detects Sox2 protein (Alexa 488) in the zebrafish neural tube by whole mount immunohistochemical analysis.
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ICC/IF
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal iScience in 2019.PMID: 31055217
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 409
$ 219
Currently in production