Western blot - Schizosaccharomyces pombe homogenates were prepared and proteins separated on a 10% SDS-PAGE gel and probed with the SpUlp2 antibody at a 1:500 dilution. A goat anti-rabbit-HRP antibody was used at a 1:5,000 dilution. Suggested starting dilution of 1:500 to 1:1,000 dilution. However, the investigator should determine the optimal dilution for a specific application such as immunohistochemistry.
Schizosaccharomyces pombe homogenates
Reacts with residues 622-635 [SNNERQSLSSGSND] of the SpUlp2 protein.
PBS containing 0.02% sodium azide.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Affinity purified IgG
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.