Reactivity of GTX00864 Staphylococcus aureus Enterotoxin A + Enterotoxin B antibody [sa-01] with various food poisoning bacteria.

Reactivity of GTX00864 Staphylococcus aureus Enterotoxin A + Enterotoxin B antibody [sa-01] with various food poisoning bacteria in immunochromatographic strip test. A mouse mono antibody was coated onto a specific area (test line) of a nitrocellulose membrane, while goat anti-mouse IgG was coated onto another specific area (control line) on the same membrane. Extract of each strain of food poisoning bacteria was mixed with the MAb (sa-01) conjugated with colloidal gold. The strip was soaked and reacted with the mixture fluid. (1) S. aureus, (2) Escherichia coli O157:H7, (3) E. coli K12, (4) Salmonella Enteritidis, (5) Campylobacter jejuni, (6) Vibrio parahaemolyticus, (7) PBS. MAb (sa-01) reacted with S. aureus, but not with other food poisoning bacteria.

WB analysis of various samples using GTX00864 Staphylococcus aureus Enterotoxin A + Enterotoxin B antibody [sa-01]. The molecular masses of enterotoxin A and B are 30 kDa and 29 kDa, respectively.
Lane 1 : Culture medium of S. aureus
Lane 2 : Purified S. aureus enterotoxin A
Lane 3 : Purified S. aureus enterotoxin B

ELISA analysis of crude extract of S. aureus using GTX00864 Staphylococcus aureus Enterotoxin A + Enterotoxin B antibody [sa-01].
Protein coating amount : 100μl (1 μg/ml) per well
Antibody amount : 100μl at indicated dilution