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Streptavidin (HRP)

Streptavidin (HRP). GTX27403
Streptavidin (HRP). GTX27403
Streptavidin (HRP). GTX27403

Cat No. GTX27403

Application WB, IHC-P, IHC-Fr, Dot, ELISA, IM
Package
500 μg ($149)
PRODUCT

Product Description

Streptavidin is a tetrameric protein capable of binding 4 biotin groups to each molecule of streptavidin. While streptavidin has identical binding properties as avidin, it lacks the glycoprotein portion of the molecule and therefore shows less non-specific binding. Streptavidin is a slightly smaller molecule with a molecular weight of approximately 53.6 kDa. The sequence of avidin only shows 30% homology with streptavidin, and anti-avidin and anti-streptavidin antibodies are not immunologically cross reactive. Horseradish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors.
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
Dot Assay dependent
ELISA Assay dependent
IM Assay dependent
Not tested in other applications.
PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate, 0.15M sodium chloride (pH 7.2).

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4º C.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Expression System

Streptomyces avidinii

Purification


This product was prepared from chromatographically purified streptavidin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Streptavidin. No reaction was observed against anti-Avidin.

Conjugation

Horseradish peroxidase(HRP)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
DATA IMAGES
Streptavidin (HRP). GTX27403

GTX27403 WB Image

Detection of Mouse anti histidine tag:biotin (GTX74967) in a Western blot analysis of a HIS tagged protein using Streptavidin:HRP (GTX27403)

Streptavidin (HRP). GTX27403

GTX27403 WB Image

Western Blot of Peroxidase Conjugated Streptavidin. Lane 1: Human IL-7. Lane 2: none. Load: 50 ng per lane. Primary antibody: Human IL-7 Biotin Conjugated antibody (GTX27403) at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase Conjugated Streptavidin at 1:40,000 for 30 min at RT. Block: 5% BLOTTO 30 min at RT. Predicted/Observed size: 17 kDa, 17 kDa for Human IL-7. Other band(s): none

Streptavidin (HRP). GTX27403

GTX27403 ELISA Image

Detection of Rabbit anti Mouse TNFα:biotin (GTX74045) in a sandwich ELISA with recombinant murine TNFα as the antigen and Rat anti Mouse TNFα as the coating antibody using Streptavidin:HRP (GTX27403)

REFERENCE
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Package List Price ($)
$ 149