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Synaptotagmin (phospho Ser309) antibody

Anti-Synaptotagmin (phospho Ser309) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50249

Cat No. GTX50249

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

ICC/IF

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:100-1:200
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 309 (G-L-S(p)-D-P) derived from human Synaptotagmin.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

synaptotagmin 1 , BAGOS , P65 , SVP65 , SYT

Cellular Localization

Cytoplasm

Background

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-Synaptotagmin (phospho Ser309) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50249

GTX50249 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50249 Synaptotagmin (phospho Ser309) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 319