HeLa whole cell extracts and HeLa exosome extract (3.5 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody [C2C3], C-term (GTX108470) diluted at 1:250. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Syntenin 1(GTX108470) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Syntenin 1 antibody [C2C3], C-term detects Syntenin 1 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Syntenin 1 antibody [C2C3], C-term (GTX108470) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Syntenin 1 antibody [C2C3], C-term detects Syntenin 1 protein at cytosol on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Syntenin 1 antibody [C2C3], C-term (GTX108470) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Syntenin 1 antibody [C2C3], C-term detects Syntenin 1 protein by immunohistochemical analysis.
Samples: Frozen Sectioned adult mouse brain.
Green: Syntenin 1 protein stained by Syntenin 1 antibody [C2C3], C-term (GTX108470) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Syntenin 1 antibody [C2C3], C-term detects Syntenin 1 protein at cytosol on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Syntenin 1 antibody [C2C3], C-term (GTX108470) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Syntenin 1 antibody [C2C3], C-term detects Syntenin 1 protein by immunohistochemical analysis.
Samples: Frozen Sectioned adult mouse brain.
Green: Syntenin 1 protein stained by Syntenin 1 antibody [C2C3], C-term (GTX108470) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Syntenin 1 antibody detects Syntenin 1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Syntenin 1 antibody (GTX108470) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.