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T-Plastin antibody [GT3310]

KO/KD
Anti-T-Plastin antibody [GT3310] used in Western Blot (WB). GTX632482
Anti-T-Plastin antibody [GT3310] used in Western Blot (WB). GTX632482
Anti-T-Plastin antibody [GT3310] used in IHC (Paraffin sections) (IHC-P). GTX632482
Anti-T-Plastin antibody [GT3310] used in IHC (Paraffin sections) (IHC-P). GTX632482
Anti-T-Plastin antibody [GT3310] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632482

Cat. No. GTX632482

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT3310

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all T-Plastin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

293T , A431 , HeLa

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human T-Plastin. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888316

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

plastin 3 , BMND18 , T-plastin

Background

Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010]

Database

Research Area

DATA IMAGES

Anti-T-Plastin antibody [GT3310] used in Western Blot (WB). GTX632482

GTX632482 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with T-Plastin antibody [GT3310] (GTX632482) diluted at 1:1000.

Anti-T-Plastin antibody [GT3310] used in Western Blot (WB). GTX632482

GTX632482 WB Image

T-Plastin antibody detects T-Plastin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with T-Plastin antibody (GTX632482) diluted at a dilution of 1:1000.

Anti-T-Plastin antibody [GT3310] used in IHC (Paraffin sections) (IHC-P). GTX632482

GTX632482 IHC-P Image

T-Plastin antibody [GT3310] detects T-Plastin protein at cytoplasm in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
T-Plastin antibody [GT3310] (GTX632482) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-T-Plastin antibody [GT3310] used in IHC (Paraffin sections) (IHC-P). GTX632482

GTX632482 IHC-P Image

T-Plastin antibody [GT3310] detects T-Plastin protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
T-Plastin antibody [GT3310] (GTX632482) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-T-Plastin antibody [GT3310] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632482

GTX632482 ICC/IF Image

T-Plastin antibody [GT3310] detects T-Plastin protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: T-Plastin protein stained by T-Plastin antibody [GT3310] (GTX632482) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

Application Reference

Schenk LK et al. FASEB J 2017; (Epub) Angiotensin II regulates phosphorylation of actin-associated proteins in human podocytes.
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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations