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T-Plastin antibody [GT3310]

KO/KD
Anti-T-Plastin antibody [GT3310] used in Western Blot (WB). GTX632482
Anti-T-Plastin antibody [GT3310] used in Western Blot (WB). GTX632482
Anti-T-Plastin antibody [GT3310] used in IHC (Paraffin sections) (IHC-P). GTX632482
Anti-T-Plastin antibody [GT3310] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632482
Anti-T-Plastin antibody [GT3310] used in IHC (Paraffin sections) (IHC-P). GTX632482

Cat No. GTX632482

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT3310

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)
See all T-Plastin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

Positive Control

293T , A431 , HeLa

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human T-Plastin. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888316

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

plastin 3 , BMND18 , T-plastin

Background

Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010]

Database

Research Area

DATA IMAGES

Anti-T-Plastin antibody [GT3310] used in Western Blot (WB). GTX632482

GTX632482 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with T-Plastin antibody [GT3310] (GTX632482) diluted at 1:1000.

Anti-T-Plastin antibody [GT3310] used in Western Blot (WB). GTX632482

GTX632482 WB Image

T-Plastin antibody detects T-Plastin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with T-Plastin antibody (GTX632482) diluted at a dilution of 1:1000.

Anti-T-Plastin antibody [GT3310] used in IHC (Paraffin sections) (IHC-P). GTX632482

GTX632482 IHC-P Image

T-Plastin antibody [GT3310] detects T-Plastin protein at cytoplasm in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
T-Plastin antibody [GT3310] (GTX632482) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-T-Plastin antibody [GT3310] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632482

GTX632482 ICC/IF Image

T-Plastin antibody [GT3310] detects T-Plastin protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: T-Plastin protein stained by T-Plastin antibody [GT3310] (GTX632482) diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-T-Plastin antibody [GT3310] used in IHC (Paraffin sections) (IHC-P). GTX632482

GTX632482 IHC-P Image

T-Plastin antibody [GT3310] detects T-Plastin protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
T-Plastin antibody [GT3310] (GTX632482) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Schenk LK et al. FASEB J 2017; (Epub) Angiotensin II regulates phosphorylation of actin-associated proteins in human podocytes.
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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169
Custom Conjugations