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T-Plastin antibody

Anti-T-Plastin antibody used in IHC (Paraffin sections) (IHC-P). GTX33552
Anti-T-Plastin antibody used in Immunoprecipitation (IP). GTX33552
Anti-T-Plastin antibody used in Western Blot (WB). GTX33552

Cat No. GTX33552

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IP

Reactivity

Human
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:200-1:2000
IHC-P 1:50-1:100
IP 1:50-1:100
Not tested in other applications.

Calculated MW

71 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human PLS3 (NP_005023.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

plastin 3 , BMND18 , T-plastin

Cellular Localization

Cytoplasm

Background

Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010]

Database

Research Area

DATA IMAGES

Anti-T-Plastin antibody used in IHC (Paraffin sections) (IHC-P). GTX33552

GTX33552 IHC-P Image

IHC-P analysis of human stomach tissue using GTX33552 T-Plastin antibody.
Dilution : 1:100

Anti-T-Plastin antibody used in Immunoprecipitation (IP). GTX33552

GTX33552 IP Image

IP analysis of HeLa cells using GTX33552 T-Plastin antibody.
IP reaction : 200μg extracts / 3μg antibody antibody
Dilution : 1:1000

Anti-T-Plastin antibody used in Western Blot (WB). GTX33552

GTX33552 WB Image

WB analysis of various sample lysates using GTX33552 T-Plastin antibody.
Loading : 25ug per lane
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329