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TAF12 antibody

Anti-TAF12 antibody used in Immunoprecipitation (IP). GTX130863
Anti-TAF12 antibody used in Western Blot (WB). GTX130863

Cat No. GTX130863

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

293T , A431 , HepG2 , HeLa , HeLa nuclear extract

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Dog, Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TAF12. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886369

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

TATA-box binding protein associated factor 12 , TAF2J , TAFII20

Cellular Localization

Nucleus

Background

Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TAF12 antibody used in Immunoprecipitation (IP). GTX130863

GTX130863 IP Image

Immunoprecipitation of TAF12 protein from 293T whole cell extracts using 5 μg of TAF12 antibody (GTX130863).
Western blot analysis was performed using TAF12 antibody (GTX130863).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-TAF12 antibody used in Western Blot (WB). GTX130863

GTX130863 WB Image

TAF12 antibody detects TAF12 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with TAF12 antibody (GTX130863) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf