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TAF172 antibody [C3], C-term

Anti-TAF172 antibody [C3], C-term used in Immunoprecipitation (IP). GTX106359
Anti-TAF172 antibody [C3], C-term used in Western Blot (WB). GTX106359

Cat No. GTX106359

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

207 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human TAF172. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949764

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

B-TFIID TATA-box binding protein associated factor 1 , MOT1 , TAF(II)170 , TAF172 , TAFII170

Cellular Localization

Nucleus

Background

Initiation of transcription by RNA polymerase II requires the assistance of TATA box-binding protein (TBP; MIM 600075) and TBP-associated factors, or TAFs (e.g., TAF2B; MIM 604912), in 2 distinct complexes, TFIID and B-TFIID. The TFIID complex is composed of TBP and more than 8 TAFs. However, the majority of TBP is present in the B-TFIID complex, which is composed of TBP and TAFII170, also called TAF172, and has DNA-dependent ATPase activity.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-TAF172 antibody [C3], C-term used in Immunoprecipitation (IP). GTX106359

GTX106359 IP Image

TAF172 antibody immunoprecipitates TAF172 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of TAF172 protein by 2 μg of TAF172 antibody (GTX106359) 5% SDS-PAGE The immunoprecipitated TAF172 protein was detected by TAF172 antibody (GTX106359) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-TAF172 antibody [C3], C-term used in Western Blot (WB). GTX106359

GTX106359 WB Image

Sample(30μg whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: HeLa S3 (GTX14654)
E: Hep G2 (GTX27900)
F: MOLT4 (GTX27912)
G: Raji (GTX27908)
5% SDS PAGE
GTX106359 diluted at 1:1000

REFERENCE

There are currently no references for TAF172 antibody [C3], C-term (GTX106359). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for TAF172 antibody [C3], C-term (GTX106359). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169